| 蟾毒灵抑制人白血病多药耐药K562/VCR细胞株增殖的实验研究 |
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| 引用本文： | 方凡夫,张亚妮,顾伟.蟾毒灵抑制人白血病多药耐药K562/VCR细胞株增殖的实验研究[J].中药新药与临床药理,2010,21(3). |
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| 作者姓名： | 方凡夫 张亚妮 顾伟 |
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| 作者单位： | 第二军医大学长海医院中医科,上海,200433 |
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| 基金项目： | 国家自然科学基金，上海市青年科技启明星计划 |
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| 摘 要： | 目的探讨蟾毒灵对人白血病多药耐药K562/VCR细胞株增殖抑制的作用及其可能的机制。方法采用MTT比色法测定蟾毒灵对多药耐药K562/VCR细胞株的增殖抑制率,倒置显微镜及Wright-Giemsa染色法观察细胞形态学改变,流式细胞术(FCM)分析蟾毒灵对K562/VCR细胞株细胞周期分布的影响,免疫细胞化学法检测耐药细胞中胸苷酸合酶(TS)表达的变化。结果蟾毒灵能够显著抑制人白血病多药耐药K562/VCR细胞株的增殖,0.01μmol/L的蟾毒灵即可诱导耐药细胞出现典型的细胞凋亡形态学特征,FCM细胞周期分析显示G2/M期细胞比率减少、"亚G1期"细胞增多,0.001μmol/L的蟾毒灵可显著下调K562/VCR多药耐药细胞中TS蛋白的表达。结论人白血病多药耐药K562/VCR细胞株对蟾毒灵无交叉耐药性,蟾毒灵可诱导耐药细胞周期阻滞和细胞凋亡,下调TS蛋白可能是蟾毒灵抑制多药耐药细胞株增殖的机制之一。
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| 关 键 词： | 蟾毒灵 白血病 多药耐药 细胞凋亡 胸苷酸合酶 |
Experimental Study on Bufalin Inhibiting the Proliferation of Multidrug-resistance Human Leukaemia Cell Line K562/VCR |
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| FANG Fanfu,ZHANG Yani,GU Wei.Experimental Study on Bufalin Inhibiting the Proliferation of Multidrug-resistance Human Leukaemia Cell Line K562/VCR[J].Traditional Chinese Drug Research & Clinical Pharmacology,2010,21(3). |
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| Authors: | FANG Fanfu ZHANG Yani GU Wei |
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| Institution: | FANG Fanfu,ZHANG Yani,GU Wei(Department of Traditional Chinese Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China) |
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| Abstract: | Objective To investigate the effect of bufalin on the proliferation of multidrug-resistant human leukaemia K562/VCR cell line and to explore its possible mechanism.Methods Proliferative inhibition rate was determined by MTT colorimetric assay.Cell morphology was observed by inverted microscope and Wright-Giemsa staining method.Cell cycle was analyzed with flow cytometry (FCM).Expression of thymidylate synthase (TS) protein was measured by immunocytochemistry.Results Bufalin remarkably inhibited the prolifer... |
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| Keywords: | Bufalin Leukaemia Multidrug resistance Cell apoptosis Thymidylate synthase |
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