The action of phosphotriose,phospho-enolpyruvic acid and the preparation ZSC (zymostimulator cordis) on cardiac muscle

Summary Preparations of the sodium salt of triose phosphate, obtained by biochemical means, as well as those of the sodium salt of phospho-enolpyruvic acid (PEP) obtained both biochemically and by chemical synthesis were studied in respect to their action upon the fatigued frog's heart (by Straub's method). Synthetic PEP was found to be inactive, while triose phosphate and biochemically obtained PEP exercise a stimulating effect on the heart similar by its character and its effective dose to the action of the sodium salt of fructose-1,6-diphosphate (DPF).Treatment of triose phosphate preparations (obtained biochemically) and those of PEP with charcoal at various pH, and their subsequent examination along with that of the eluted fluid (wash off) were investigated. It was demonstrated that the action of the preparations on the fatigued heart is, at least in its main part, associated with the presence of the low quantities of zymostimulator cordis (ZSC) as admixture. ZSC is a uridine derivative close or identical to uridinediphosphate (UDP) formerly isolated by the authors from yeast and sodium-DPF preparations.Presented by Active Member AMN SSSR S. E. Severin