Effect of genetic polymorphism of GSTM1 and GSTT1 genotypes on cytogenetic biomarkers among coaltar workers |
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| Authors: | Kumar Anil Yadav Anita Giri Shiv Kumar Dev Kapil Gautam Sanjeev Kumar Gupta Ranjan Aggarwal Neeraj |
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| Affiliation: | aDepartment of Biotechnology, Kurukshetra University Kurukshetra, Haryana, India;bDepartment of Biochemistry, Kurukshetra University Kurukshetra, Haryana, India;cDepartment of Microbiology, Kurukshetra University Kurukshetra, Haryana, India |
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| Abstract: | Chromosomal aberrations (CAs) in peripheral blood lymphocytes and micronuclei (MN) in exfoliated buccal cells have been used for decades as cytogenetic biomarkers to investigate genotoxicity among occupationally or environmentally exposed population. In our study, we investigated the association of increased cytogenetic damage with genetic polymorphism in glutathione-S transferase genotypes among occupationally exposed 115 coaltar workers and 105 unexposed controls. We found higher mean value of chromosome aberrations (chromatid type-2.01 ± 1.76; chromosomal type-2.22 ± 1.73) and buccal micronuclei (BMN-7.10 ± 1.56) in exposed subjects when compared to referents (chromatid type-0.82 ± .51; chromosomal type-0.87 ± .54; BMN-5.09 ± 2.88). We observed that individuals having null genotype of GSTM1 and GSTT1 have significantly higher frequency of CAs and MN. Despite of small sample size, our findings suggest a significant association between polymorphism of glutathione-S transferase genotypes and cytogenetic biomarkers which are considered as early effects of genotoxic carcinogens. |
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| Keywords: | Chromosome aberrations Genetic polymorphism Glutathione-S transferase Buccal micronuclei Peripheral blood lymphocytes |
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