| 转染XIAP基因对丝裂霉素诱导膀胱癌细胞凋亡的影响 |
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| 引用本文： | 汪良,曾甫清,陈方敏,刘迎. 转染XIAP基因对丝裂霉素诱导膀胱癌细胞凋亡的影响[J]. 临床泌尿外科杂志, 2004, 19(4): 226-228 |
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| 作者姓名： | 汪良 曾甫清 陈方敏 刘迎 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院泌尿外科,武汉,430022;华中科技大学同济医学院附属协和医院泌尿外科,武汉,430022;华中科技大学同济医学院附属协和医院泌尿外科,武汉,430022;华中科技大学同济医学院附属协和医院泌尿外科,武汉,430022 |
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| 基金项目： | 国家自然科学基金资助项目 (No:30 2 7130 1) |
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| 摘 要： | 目的：探讨XIAP基因对低剂量丝裂霉素C(MMC)诱导膀胱癌细胞凋亡的影响。方法：脂质体介导XIAP基因转染膀胱癌T24细胞3天后．用逆转录聚合酶链反应(RT-PCR)检测XIAP基因的表达．用低剂量MMC诱导细胞凋亡启动，用MTT比色法分析检测癌细胞生长活性．用3末端脱氧核苷酰转移酶介导生物素标记法检测细胞凋亡率。结果：各组癌细胞在低剂量MMC的诱导下发生凋亡、与单用MMC组比较．转染XIAP基因的癌细胞组的凋亡率显著降低。结论：XIAP基因在癌细胞中的过度表达可以明显降低化疗药物诱导的膀胱癌细胞凋亡，其可能在膀胱癌多药耐药中起一定作用。
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| 关 键 词： | 膀胱肿瘤 XIAP基因 丝裂霉素C 细胞凋亡 |
| 文章编号： | 1001-1420(2004)04-0226-03 |
| 修稿时间： | 2003-11-19 |
Experimental study on XIAP blocking the induction of mitomycin C on bladder cancer apoptosis |
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| WANG Liang ZENG Fuqing CHEN Fangmin LIU Ying. Experimental study on XIAP blocking the induction of mitomycin C on bladder cancer apoptosis[J]. Journal of Clinical Urology, 2004, 19(4): 226-228 |
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| Authors: | WANG Liang ZENG Fuqing CHEN Fangmin LIU Ying |
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| Affiliation: | WANG Liang 1 ZENG Fuqing 1 CHEN Fangmin 1 LIU Ying 1 |
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| Abstract: | Objective:To explore the effect of XIAP gene on the apoptosis induced by low-dose mitomycin C on the bladder cancer cell line T24.Method:The XIAP gene was transferred into bladder cancer cell line T24 under the induction of liposome. The intrinsic XIAP level was detected by RT-PCR. Low dose mitocycin C induces the apoptosis of T24 cells. The invitro cellular growth activities were estimated by MTT colorimetry; the apoptosis rate was assayed by TUNEL methods.Result:Incompare with the control cells, the apoptosis rate of T24 cells induced by mitomycin C was decreased by trasfected XIAP gene.Conclusion:The XIAP could decrease the apoptosis rate of T24 cells induced by mitomycin C, and it could be a mechanism of multidrug resistant bladder cancer. |
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| Keywords: | Bladder neoplasms XIAP gene Mitomycin C Apoptosis |
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