| 石棉暴露下内质网应激在肺泡上皮细胞凋亡中的作用机制 |
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| 引用本文： | 周煦,刘刚. 石棉暴露下内质网应激在肺泡上皮细胞凋亡中的作用机制[J]. 现代医院, 2014, 0(8): 9-10 |
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| 作者姓名： | 周煦 刘刚 |
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| 作者单位： | 广东医学院,广东湛江524023 |
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| 基金项目： | 国家自然科学基金（编号：81172615）,广东省自然科学基金（编号：S2012010008299）,广东医学院青年基金项目（编号：Q2012016） |
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| 摘 要： | 目的探讨石棉暴露下内质网应激在细胞凋亡中的作用机制。方法应用石棉处理A549细胞,免疫荧光染色免疫法和免疫印迹法观察内质网应激(ERS)相关蛋白与促凋亡基因的变化。结果石棉暴露下,ERS相关蛋白Bip、IRE-1α和GRP94,以及促凋亡基因BAX、BAK蛋白表达均上调,且与石棉暴露时间呈正相关。结论内质网应激参与了石棉诱导的细胞凋亡。
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| 关 键 词： | 石棉 内质网应激 凋亡 |
THE MECHANISM OF ENDOPLASMIC RETICULUM STRESS INDUCING ALVEOLAR EPI-THELIAL CELLS APOPTOSIS UNDERLYING ASBESTOS EXPOSURE |
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| ZHOU Xu,LIU Gang. THE MECHANISM OF ENDOPLASMIC RETICULUM STRESS INDUCING ALVEOLAR EPI-THELIAL CELLS APOPTOSIS UNDERLYING ASBESTOS EXPOSURE[J]. Modern Hospital, 2014, 0(8): 9-10 |
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| Authors: | ZHOU Xu LIU Gang |
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| Affiliation: | (Guangdong Medical College, Zhanjiang, Guangdong, 524023 PRC) |
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| Abstract: | Objective To investigate the role of endoplasmic reticulum stress （ ERS） in asbestos-induced Alveolar epithelial cell apoptosis.Methods A549 cells were treated with asbestos to observe the expression of ERS proteins and apop-tosis gene by immunofluorescence staining and western blotting.Results Under asbestos exposure, the expression of Bip, GRP94, IRE-1α, BAX and BAK are up-regulated.There is a positive correlativity of the expression of proteins to the ex-posure time of asbestos.Conclusion Endoplasmic reticulum stress takes part in asbestos-induced apoptosis. |
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| Keywords: | Asbestos Endoplasmic Reticulum Stress Apoptosis |
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