三起食物中毒来源副溶血弧菌毒力基因检测分析

目的分离3起食物中毒患者粪便及食物加工工具样本中的病原菌，对分离菌株进行表型和毒力基因鉴定。方法可疑病原菌的分离与鉴定按照国标GB/T 4789-2003和GB/T 4789.7-2008规定的方法进行；用PCR方法对检出的副溶血弧菌(IVibrioparahaemolyticus,/IVp)进行Vp种属特异基因(320 bp)和毒力基因Itdh/I( 耐热溶血素434 bp)、Itrh/I( 耐热相关溶血素250 bp) 检测；用环介导恒温扩增(LAMP)方法对分离株Itlh/I( 不耐热溶血素229 bp)基因进行检测。结果从3起食物中毒的患者、厨师粪便和食品加工环节共27件样品中检出7株Vp，其中加工环节熟食冷菜间刀具涂抹物中检出1株，患者粪便中检出6株。3起食物中毒7株菌血清型分属为O10∶K66、O2∶K3、O1∶K33, 每起食物中毒分离株的生物学性状、血清型和药敏试验结果均一致。7株Vp均产生神奈川现象（KP）、出现相同Vp种属特异基因条带、均检出Itlh/I毒力基因，但Itdh/I、Itrh/I基因则检测为阴性。结论3起食物中毒均为Itlh/I+/Itdh/I-/Itrh/I-不同血清型Vp污染引起。

Virulence gene detection of Vibrio parahaemolyticus in 3 food poisoning events

Objective To isolate pathogenes from stool samples of patients and other samples collected from food-processing tools in 3 food poisoning events,and identify the type and virulence gene of pathogens isolated. MethodsSuspect pathogen was isolated and identified in accordance with National Standard GB/T 4789-2003 and GB/T 4789.7 -2008;The detected Vibrio parahaemolyticus was tested for species-specific gene (320 bp),thermostable direct hemolysin gene (tdh 434 bp),tdh-related hemolysin gen (trh 250 bp) by PCR ...