HPLC测定白花前胡蜜炙前后3种香豆素类成分的含量

 目的 对前胡蜜炙前后3种香豆素类成分进行比较研究。方法 HPLC同时测定3种香豆素类成分白花前胡甲素、白花前胡乙素、白花前胡E素的含量,Agilent TC-C₁₈ (2)柱,流动相为甲醇-水（3︰1）,检测波长320 nm,流速0.8 mL·min-1,柱温35 ℃。结果 3个成分的线性关系较好（r>0.999 6）,测定范围分别为0.204 4~2.044, 0.077 6~0.776,0.077 2~0.772 μg,平均回收率分别为101.66%,97.45%,97.79%。前胡不同饮片中3种香豆素类成分含量有差异。结论 所建立的方法可用于同时测定前胡不同饮片中3种香豆素的含量,为前胡不同饮片质量评价标准的制定提供了科学依据。

Simultaneous Analysis of Three Coumarins in Processed Pieces from Peucedanum praeruptorum by HPLC

OBJECTIVE To compare the contents of coumarin in the different processed products from Peucedanum praeruptorum Dunn. METHODS (±)- Praeruptorin A, (±)-Praeruptorin B and (＋)-Praeruptorin E were determined simultaneously by HPLC. The separation was performed on a Agilent TC-C₁₈ (2) column at 35 ℃ with MeOH-H₂O(3︰1) as the mobile phrase. The detection wavelength was set at 320 nm and the flow rate was 0.8 mL·min-1. RESULTS The standard curves for the three components were linear within the ranges of 0.204 4-2.044 μg（r=0.999 9,n=6）, 0.077 6-0.776 μg（r=0.999 6,n=6）,0.077 2-0.772 μg（r=0.999 9,n=6）, and the average recoveries were 101.66%, 97.45% and 97.79%, respectively. CONCLUSION The method can be used for evaluating the quality in different processed pieces of P. praeruptorum.