NSCL/P患儿异常表达miRNAs的生物信息学分析 |
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引用本文: | 苏艳国,孟琰,孙长生,施磊,强冬霞,赵尔杨.NSCL/P患儿异常表达miRNAs的生物信息学分析[J].实用口腔医学杂志,2016(6):805-809. |
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作者姓名: | 苏艳国 孟琰 孙长生 施磊 强冬霞 赵尔杨 |
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作者单位: | 哈尔滨医科大学附属第一医院口腔医学院口腔组织病理科,150001 |
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基金项目: | 黑龙江省教育厅基金(12511220),李嘉诚基金-全国“重生行动”科研基金 |
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摘 要: | 目的::利用芯片杂交技术筛选出非综合征性唇腭裂( NSCL/P)患儿组织中异常表达的microRNAs ( miRNAs),并进行系统的生物信息学分析,预测其参与的生物学过程及信号通路。方法:收集4例健康新生儿的脐带组织和4例2岁以内NSCL/P患儿唇腭组织,分别进行miRNAs芯片杂交的比对研究,筛选出异常表达miRNAs,并将其分别通过TARGETSCAN-VERT、MIRDB和RNA22-HSA 3个数据库进行生物信息学预测并取交集得到靶基因,进行基因功能富集分析( gene ontology)和生物通路富集分析( pathway enrichment)。结果:样本中检测出异常表达的miRNAs 254条(上调表达181条,下调表达73条, P<0.05)。对异常表达的miRNAs进行生物信息学预测并取交集得到靶基因5029个。异常表达的miRNAs靶基因富集于解剖结构的发育、细胞黏附、细胞凋亡、细胞分化和细胞迁移等多个生物学过程;异常表达的miRNAs靶基因信号通路富集于Wnt、 mTOR、cGMP-PKG、TGFβ、PI3K-Akt等信号通路。结论:NSCL/P异常表达miRNAs预测出的靶基因富集于多个信号通路和生物学功能,其相关信号通路与生物学功能提示基因与环境因素能够影响NSCL/P的形成。
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关 键 词: | miRNAs 非综合征性唇腭裂(NSCL/P) 生物信息学 靶基因 信号通路 |
Bioinformatics analysis of dysregulated miRNAs in the tissue of children with nonsyndromic cleft lip and/or cleft palate |
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Abstract: | Objective:To screen the differentially expressed miRNAs in umbilical cord tissue of children with nonsyndromic cleft lip and/or cleft palate( NSCL/P) using miRNA microarray and comprehensive bioinformatics analysis for the prediction of related the bio-logical process and signaling pathways. Methods:Umbilical cord tissues of 4 cases of healthy newborns' and 4 lip or palate tissues of 4 cases with NSCL/P without other disease aged younger than 2 years were collected. The differentially expressed miRNAs were screened by miRNA microarray. Targets of dysrugulated miRNAs were predicted by TARGETSCAN-VERT, MIRDB and RNA22-HSA. All the gene sets were analyzed by gene ontology and pathway enrichment. Results: MiRNA microarray demonstrated that 254 miRNAs were dysregulated(181 miRNAs were up-regulated and 73 downregulated,P <0. 05). The dysregulated miRNAs targets contained 5029 genes. The dysregulated miRNAs targets were enriched in anatomical structure development,cell adhesion,cell proliferation,cell motili-ty and other biological processes. The dysregulated miRNAs targets were enriched in Wnt, mTOR, cGMP-PKG, TGFβ, PI3K-Akt and other signaling pathways. Conclusion:The target genes set of miRNAs are enriched in multiple biological processes and signaling path-ways related to NSCL/P, which indicate that genetic and environmental factors may influence the development process of NSCL/P. |
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Keywords: | miRNA NSCL/P Bioinformatic analysis Target gene Signaling pathway |
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