表面梯度涂层纯钛植入体骨结合和骨诱导能力的动物实验研究

目的：观察纯钛植入体表面用低温烧结技术生成羟基磷灰石/生物玻璃( nHA/BG)梯度涂层植入动物体内的骨结合和骨诱导能力。方法：将nHA/BG按比例混合，应用低温烧结技术在圆柱形纯钛植入体表面形成梯度涂层，模拟体液浸泡后产生活性纳米结构。将梯度涂层实验植入体和纯钛对照植入体分别植入12只新西兰兔股骨髁。术后不同时间行X线摄片，切取标本；硬组织切片，用四环素荧光标记观察植入体周围新生骨形成量；苦味酸-品红染色观察植入体-骨界面骨沉积和结合情况。结果：低温烧结和模拟体液浸泡制备的nHA/BG梯度涂层为纳米化多孔粗糙结构，动物实验示，梯度涂层植入体与周围新生骨明显增多，骨整合良好。结论：梯度涂层和模拟体液浸泡制备的植入体涂层有良好的骨结合和骨诱导能力。

The osteointergration and osteoinduction of titanium implant with nHA/BG gradient coating in rabbits

Objective: To investigate the osteointegration and osteoinduction of nano hydroxyapatite/bioglass ( nHA/BG ) gradient nanofilm on the surface of titanium ( Ti) prepared by hypotherm sintering and plastic deformation. Methods:Hypotherm sintering was used to produce nHA/BG gradient coating followed by soaking in the simulated body fluid. Ti implants with gradient coatings were planted in femoral condyles at one side of 12 New Zealand rabbits and the untreated Ti implants were planted at the other side as the controls. 1, 3 and 6 months after implantation, the animals were sacrificed after X-ray examination and the tissues around the implants from the 3 month group were used for the preparation of hard tissue section and ground section. New bone formation was observed by tetracycline fluorescence staining. Von Gieson staining was used to observe the osteointegration at the interface between bone and im-plant. Results:The gradient coatings were porous and composed of irregular rod-like nano-HA crystals. Animal study showed well es-tablished osteointegration between the gradient coating and more novel bone was found around the implants with gradient coatings. Conclusion:Osteointegration and ostioinduction of Ti implant can be enhanced by nanostructured surface with gradient coatings of nHA/BG.