氯已定在大鼠牙周炎模型建立过程中的抑制作用

目的：探讨不同浓度的氯已定在牙龈卟啉单胞菌 W83（P．gingivalis W83）引起的大鼠牙周炎模型中的抑制作用。方法：采用 P．gingivalis W83灌饲和钢丝结扎牙颈部的方法建立大鼠牙周炎模型，在建立模型的过程中，采用0．05％、0．1％、0．2％和0．5％的氯已定冲洗大鼠牙周袋及口腔黏膜，4周后，绝对实时定量 PCR 检测各组大鼠牙周袋内的 P．gingivalis W83拷贝数，扫描电镜观察各组大鼠牙齿表面的 P．gingivalis W83定植情况。免疫组织化学技术检测大鼠牙龈组织中 TNF-α的表达（P ＜0．05）。结果：0．2％和0．5％的氯已定能够有效减少大鼠牙周袋内及牙齿表面的 P．gingivalis W83数量（P＜0．05）；0．1％～0．5％的氯已定能够减少大鼠牙龈组织中 TNF-α的表达（P ＜0．05）结论：0．1％～0．2％的氯已定能够抑制大鼠慢性牙周炎的进展。

The inhibitory effects of chlorhexidine in the development of rat periodontitis models

Objective:To investigate the inhibitory effects of different concentrations of chlorhexidine in the development of peri-odontitis models in rats.Methods:periodontitis models were established by the ligation of bilateral first molars and orally challenge with P.gingivalis W83.0.05%,0.1%,0.2% and 0.5% chlorhexidine were used to wash the periodontal pocket and oral mucosa of the rats.4 weeks later,absolute real time quantitative PCR was used to count the copy of P.gingivalis W83 in rat periodontal pockets.Scanning electron microscopy was used to observe the distribution of P.gingivalis W83 on rat teeth surface.Immunohisto-chemical technique was used to detect the expression of TNF-αin gingival tissue of the rats.Results:0.2% and 0.5% chlorhexi-dine reduced the copy of P.gingivalis W83 on teeth surface and in periodontal pockets (P <0.05);0.1% -0.5% chlorhexidine reduced the expression of TNF-αin gingival tissue (P <0.05).Conclusion:0.1% -0.2% chlorhexidine can inhibit the develop-ment of chronic periodontitis in rats.