牙周膜干细胞中乙酰基转移酶MORF调控成骨的研究 |
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引用本文: | 袁林,孙晋,程峰,杨征毅,曹依娜,潘广嗣,钱钧,何恩亮,王涵. 牙周膜干细胞中乙酰基转移酶MORF调控成骨的研究[J]. 实用口腔医学杂志, 2016, 0(6): 778-782. DOI: 10.3969/j.issn.1001-3733.2016.06.007 |
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作者姓名: | 袁林 孙晋 程峰 杨征毅 曹依娜 潘广嗣 钱钧 何恩亮 王涵 |
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作者单位: | 广州医科大学附属第一医院口腔科,510140 |
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基金项目: | 广州市科信局科技惠民专项资助(2014Y2-00058),广州市属高校科研计划科技类一般项目(2012C096) |
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摘 要: | 目的::比较正常与炎症来源牙周膜干细胞( H-PDLSCs与P-PDLSCs)中乙酰基转移酶MORF表达水平的差异及其对PDLSCs分化的调控。方法:有限稀释法培养H-PDLSCs与P-PDLSCs, LPS、 TNF-α、IL-β及三者混合物体外模拟炎症微环境诱导H-PDLSCs ( IP-PDLSCs );基因与蛋白检测方法对比2种来源 PDLSCs 中 MORF 的表达水平;蛋白检测方法检测 IP-PDLSCs中MORF的表达水平;基因、蛋白检测与茜素红染色方法对比H-PDLSCs及下调MORF基因后的PDLSCs成骨基因表达的差异。结果:与 H-PDLSCs 相比,P-PDLSCs 中 MORF 的表达显著下降(P <0.05);IP-PDLSCs 中 MORF 的表达下降;MORF基因被下调后,PDLSCs成骨能力下降(P<0.05)。结论:牙周炎及炎症微环境会导致PDLSCs中MORF表达的下降,从而使其成骨分化受到抑制。
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关 键 词: | 牙周膜干细胞(PDLSCs) 牙周炎 乙酰基转移酶 成骨分化 |
Acetyltransferase MORF regulates osteogenic differentiation potential of periodontal ligament stem cells |
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Abstract: | Objective:To compare acetyltransferase MORF level in periodontal ligament stem cells( PDLSCs) derived from healthy individuals ( H-PDLSCs) with those derived from the individuals with periodontitis ( P-PDLSCs ) . And to determine the effect of MORF on the osteogenic differentiation potential of PDLSCs. Methods: Human H-PDLSCs and P-PDLSCs were cultured and cloned with limited dilution method. H-PDLSCs were stimulated by LPS, TNF-α, IL-β and the mix of the 3 inflammatory factors to imitate inflammatory environment ( IP-PDLSCs ) . Quantitative RT-PCR and Western Blot were applied to examine different expression of MORF in H-PDLSCs and P-PDLSCs. Western Blot was applied to detect expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red staining were applied to determine osteogenic differentiation potential of H-PDLSCs with MORF knock-down. Results:Quantitative RT-PCR and Western Blot showed lower expression of MORF in P-PDLSCs compared with H-PDLSCs( P<0. 05). Western Blot revealed lower expression of MORF in IP-PDLSCs. Quantitative RT-PCR, Western Blot and alizarin red stai-ning indicated osteogenic differentiation potential was inhibited in H-PDLSCs with MORF knockdown(P<0. 05). Conclusion: Peri-odontitis can suppress the expression of MORF in PDLSCs and inhibite the osteogenic differentiation potential of PDLSCs. |
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Keywords: | Periodontal ligament stem cells( PDLSCs) Periodontitis Acetyltransferase Osteogenic differentiation |
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