| 孕晚期羊水细胞的荧光原位杂交 |
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| 引用本文： | 游泽山,黄珈,陈建生,陈宝江,王彩玲.孕晚期羊水细胞的荧光原位杂交[J].中华围产医学杂志,2006,9(1):18-20. |
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| 作者姓名： | 游泽山 黄珈 陈建生 陈宝江 王彩玲 |
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| 作者单位： | 510080,广州,中山大学附属第一医院妇产科 |
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| 基金项目： | 广东省计划生育委员会基金（200105） |
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| 摘 要： | 目的前瞻性研究孕28周以后羊水细胞的荧光原位杂交。方法采用盲法，对孕28周后需进行产前诊断的32例孕妇在取脐带血的同时抽吸5ml羊水进行荧光原位杂交。结果32例孕妇发现染色体数目异常6例（18三体3例，21三体2例，47XYY1例）。且羊水细胞荧光原位杂交结果与脐带血染色体核型分析的结果一致，符合率达100％。结论孕28周后羊水细胞荧光原位杂交，对常见的染色体数目异常诊断准确性高，有一定的临床推广价值。
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| 关 键 词： | 羊膜腔穿刺术 妊娠末期 原位杂交 荧光 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2004年11月22 |
Interphase FISH of amniocytes in the third trimester |
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| YOU Ze-shan, HUANG Jia, CHEN Jian- sheng ,et al..Interphase FISH of amniocytes in the third trimester[J].Chinese Journal of Perinatal Medicine,2006,9(1):18-20. |
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| Authors: | YOU Ze-shan HUANG Jia CHEN Jian- sheng |
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| Institution: | YOU Ze-shan, HUANG Jia, CHEN Jian- sheng , et al. |
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| Abstract: | Objective To study the interphase FISH of cells from aminotic fluid in the third trimester prospectively. Methods Amniotic fluid (5ml) was obtained in 32 pregnant women in the third trimester who underwent cordocentesis for karyotyping. FISH was performed on uncultured am niocytes and the results were compared with karyotypes. Results Abnormal aneuploid were identified in 6 cases among the 32 women. The FISH results were consistent with the karyotypes. Conclusions Interphase FISH in third-trimester amniocentesis can provide an accurate clinical diagnosis on common aneupoidies. |
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| Keywords: | Amniocentesis Pregnancy trimester third In situ hybrldization fluorescence |
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