小儿肾病综合征血清皮质醇及T细胞亚群检测的临床意义

目的通过测定肾病综合征小儿血清皮质醇和T细胞亚群来探索小儿肾痛综合征血清皮质醇水平与T细胞亚群的关系及其临床意义。方法采用放射免疫分析的方法测定小儿血清皮质醇水平，同时应用单克隆抗体技术检测小儿的T细胞亚群。结果实验两组血清皮质醇水平差异无统计学意义（P〉0．05）；然而肾病小儿CD3^＋、CD4^＋以及CD4^＋／CD8^＋均明显低于正常对照组小儿（P〈0．05），肾病组CD8^＋比例明显高于正常对照组（P〈0．05）。结论肾病小儿糖皮质激素水平虽然基本正常，但其受体数量下调，细胞免疫功能受损，这表明T细胞亚群可作为肾病综合征活动性的一个指标。

Clinical significance of meassuring serum cortisol and T lymphocyte subsets in children with nephrotic syndrome

Objective To study the clinical significance of meassuring serum cortisol and T lymphocyte subsets,and to explore the relationship between serum cortisol and T lymphocyte subsets in children with nephrotic syndrom(NS).Methods Serum cortisol was meassured by radioimmunoassay and T lymphocyte substs were determined by monoclonal antibody technique.Results The contents of serum cortisol had no difference in two groups(P>0.05);however the percentages of CD3 cell,CD4 cell and the ratio of CD4 /CD8 were significantly lower in children with NS than in normal control group(P<0.05),the percentages of CD8 cell was significantly higher in children with NS than in normal control group(P<0.05).Conclusion Because of the normal content of serum cortisol,their cellularimmunity is impaired while the quantity of glucocorticosteroid receptor(GCR)in patients with NS is down regulation.It indicates that T lymphocyte subsets can be used as an index of activity in nephrotic syndrome.