糖皮质激素及其胞浆受体拮抗剂对凋亡嗜酸粒细胞转归的影响

目的探讨糖皮质激素及糖皮质激素胞浆受体拮抗剂RU38486对A549细胞吞噬凋亡的嗜酸粒细胞的影响。方法．CD15 的嗜酸粒细胞体外培养48 h老化凋亡后加入到预先用地塞米松或RU38486干预的A549细胞中进行吞噬,光镜下对已固定染色的细胞进行吞噬率统计。放射免疫法检测A549细胞上清中白介素6和白介素8水平。结果A549细胞在地塞米松诱导后1 h吞噬能力开始升高,4 h达最高峰;加入RU38486后抑制了地塞米松所增强的吞噬能力。结论地塞米松促进A549细胞吞噬凋亡的嗜酸粒细胞是通过A549细胞糖皮质激素胞浆受体发挥作用的。

Effect of glucocorticiod and glucocorticiod cytosolic receptors antagonist on the trend of apoptotic eosinophils

[Objective] To investigate the effects of glucocorticoid and glucocorticoid cytosolic receptors antagonist RU38486 on the phagocytosis of apoptotic eosinphils by A549 cells. [Methods] After 48 hours culture, apoptotic CD15 positive eosinphils were added to A549 cells which were pretreated with dexamethasone or RU38486 for engulfment. Calculated the ratio of engulfment under the light microscope with the cells which were fixed and stained. IL-6 and IL-8 in the supernate were measured by using radioimmunoassay method. [Results] The capacity of engulfment of A549 cells started enhancing at the 1 h of preincubation with dexamethasone > reached the highest at the 4 h of preincubation. Glucocorticoid cytosolic xeceptors antagonist RU38486 inhibited the improved engulfment ability by dexametheson. [Conclusion] Dexamethesone improves the phagocyte capacity for apoptotic eosinophils of A549 cells by glucocorticoid cytosolic receptors.