尖锐湿疣患者外周血白细胞分泌肿瘤坏死因子的体外研究

目的 研究尖锐湿疣复发与个体肿瘤坏死因子(TNF-α)分泌能力之间的关系,探讨尖锐湿疣的复发遗传基础。方法 利用体外多次传代培养的B淋巴母细胞样细胞株(LCL)作为TNF产生细胞,采用生物活性检测法检测LCL在LPS刺激下分泌TNF的能力。结果 实验结果显示尖锐湿疣患者组(包括复发患者和未复发患者)LCL的TNF分泌能力与正常人对照组差异无显著性(30.14%±12.27%与34.06%±12.06%,P=0.1136);而尖锐湿疣复发组的TNF分泌能力明显低于尖锐湿疣未复发组(24.75%±7.51%与36.62%±10.96%,P=0.00016);与正常人对照组比较,尖锐湿疣复发组的TNF分泌能力明显低下(P=0.00054),尖锐湿疣未复发组则与正常人对照组差异无显著性(P=0.3517)。结论 在清除治疗后残留HPV病毒的过程中,TNF参与的细胞免疫机制可能发挥重要作用。

Secretion of Tumor Necrotic Factor of Peripheral Blood Leukocytes from Patients with Condylomata Acuminata:An In Vitro Study

Objective To investigate the correlation between the relapse of condyloma acuminatum(CA)and the potential capability of tumor necrotic factor (TNF) production of the host's peripheral blood leukocytes. Methods Forty-two CA patients and 58 normal controls were enrolled in this study. CA relapse was diagnosed clinically. EB virus-transformed B lymphoblastoid cell line(LCL)were used as TNF producing cells. The TNF producing capability of LCL was measured by bioassay using L929 (a TNF sensitive tumor cell line) as target cells. The LCL were stimulated with LPS to produce TNF. Results The average level of TNF production of LCL from all CA patients (including recurrent and non-recurrent CA patients) was similar to that of normal controls (30.14% ± 12.27 vs 34.06% ± 12.06,P=0.1136). However, the level of TNF production of LCs from recurrent CA patients was significantly less than that from non-recurrent CA patients (24.75% ± 7.51 vs 36.62% ± 10.96,P=0.00016). Compared with that of normal controls, recurrent CA patients showed a lower capability to produce TNF (24.75% ± 7.51 vs 34.06% ± 12.06,P=0.00054), whereas non-recurrent CA patients showed a similar capability to normal controls (36.62% ± 10.96 vs 34.06% ± 12.06,P=0.3517). Conclusions These results indicate that the cellular immune mechanism might play an important role in the clearance of the residual HPV from the host, in which TNF is involved.