天麻素对脊髓缺血再灌注损伤中脊髓微循环血流量的影响

目的:观察腹主动脉内灌注天麻素对兔脊髓缺血再灌注损伤(SCIRI)中脊髓微循环血流量(SCMBF)的影响。方法:60只健康新西兰大白兔随机分为正常组、模型组和天麻素低、中、高剂量组(25,50,100 mg·kg-1),除正常组外,采用肾下腹主动脉阻断法,建立SCIRI模型,分别于缺血前,再灌注15,30,60,120 min监测SCMBF变化。分别于缺血前、再灌注6,12,24 h神经功能评分(NFS)。分别于缺血前、缺血45 min,再灌注60,120 min监测血清神经特异性烯醇化酶浓度(NSE),脊髓组织丙二醛(MDA),活性氧(ROS),谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD),总抗氧化能力(T-AOC)及脊髓病理学变化。结果:与正常组比较,模型组缺血后NSE浓度,MDA和ROS明显增加(P0.01),GSH-Px,SOD及T-AOC明显降低(P0.01)。与模型组比较,缺血后天麻素组NSE,MDA和ROS明显降低(P0.01),GSH-Px,SOD及T-AOC明显升高(P0.01)。天麻素组SCMBF和脊髓灰质的病理损害程度明显轻于模型组(P0.01),NFS较模型组恢复迅速(P0.01)。结论:SCIRI中腹主动脉内灌注天麻素可通过提高脊髓抗氧化能力来减轻脊髓微循环障碍。

Effect of Gastrodin on Spinal Cord Microcirculatory Blood Flow in Spinal Cord Ischemia Reperfusion Injury

Objective: To observe the effect of gastrodin infused through abdominal aorta on spinal cord microcirculatory blood flow (SCMBF) during the spinal cord ischemia-reperfusion injury (SCIRI). Method: Totally 60 healthy New Zealand rabbits were randomly divided into the normal control group, the model group, gastrodin low, medium and high dose groups (25,50,100 mg · kg^-1). Except for the normal group, the SCIRI model was created by clamping infrarenal aorta. The changes in SCMBF were monitored before ischemia and at 15,30,60,120 min after the reperfusion. The neurologic function score (NFS) was evaluated before ischemia and at 6, 12, 24 h after the reperfusion. The level of serum neuron-specific enolase (NSE), malondialdehyde (MDA), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total anti-oxidation capacity (T-AOC) and the pathological changes in spinal cord tissues were monitored before the ischemia and at 45,60, 120 min after the reperfusion. Result: Compared with the model group, the model group showed significantly higher NSE, MDA and ROS (P <0.01) and lower GSH-Px, SOD and T-AOC (P <0.01) after ischemia. NSE, MDA and ROS were significantly lower while GSH-Px, SOD and T-AOC were distinctly higher in the gastrodin group after ischemia than that in the model group (P <0.01). The SCMBF and pathological damage in spinal cord in the model group were obviously more serious than that in the gastrodin group (P <0.01),but NFS recovered rapidly in the gastrodin group during reperfusion than that in the model group (P <0.01). Conclusion: The intra-aortic gastrodin infusion could distinctly alleviate spinal cord microcirculatory dysfunction by infusing gastrodin through abdominal aorta and improving the anti-oxidation ability of spinal cord during SCIRI.