| 单克隆抗体亲和层析法粗提HFRS病毒的初步研究 |
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| 引用本文： | 薛采芳,安献禄,陈百虹,甄荣芬,张荣庆. 单克隆抗体亲和层析法粗提HFRS病毒的初步研究[J]. 医学争鸣, 1988, 0(4) |
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| 作者姓名： | 薛采芳 安献禄 陈百虹 甄荣芬 张荣庆 |
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| 作者单位： | 第四军医大学微生物学教研室(薛采芳，安献禄，陈百虹，甄荣芬)，第四军医大学微生物学教研室(张荣庆) |
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| 基金项目： | 国家自然科学基金(3860836) |
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| 摘 要： | 用McAb亲和层析法粗提HFRS病毒81-14A株感染BHK细胞培养上清液,效果较满意。病毒抗原获得率为48.07％。经McAb亲和层析洗脱,病毒抗原,蛋白峰值基本一致。特异性试验证明洗脱物为HFRS病毒抗原。SDS—PAGE经氨银染色主要有4条区带。
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| 关 键 词： | 流行性出血热病毒 单克隆抗体 亲和层析 纯化 |
Purification of HFRS Virus by Affinity Chromatography with Monoclonal Antibody |
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| Xue Caifang,et al.. Purification of HFRS Virus by Affinity Chromatography with Monoclonal Antibody[J]. Negative, 1988, 0(4) |
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| Authors: | Xue Caifang et al. |
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| Affiliation: | Xue Caifang,et al. Department of Microbiology |
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| Abstract: | HFRS Virus strain 81-14A was propagated in the BHK cells, and the supernatant of infected cell culture was purified roughly by monoclonal antibody affinity chromatography. The result was relatively effective. The recovery of antigens of purified virus was 48.07%. No difference was found in eluting peaks of antigens and proteins of virus. The eluted fractions were identified as antigens of HFRS virus by specific EL1SA tests. SDS-PAGE analysis was carried out by silver stain and four major bands were found. The possibility of rough purification of HFRS virus by immune-adsorbent column with McAb is discussed in this paper. |
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| Keywords: | epidemic hemorrhagic fever virus) monoclonal antibody affinity chro-matography purification |
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