125I粒子植入小鼠肝脏对肝纤维化血清指标的影响

目的:观察不同剂量125I粒子植入小鼠肝脏后对肝纤维化血清指标的影响。方法:50只昆明小鼠随机分为正常对照组(A组)和不同剂量125I粒子(125I粒子放射性活度依次为0、18.5、37.0和74.0 MBq)植入组(依次为B、C、D、E组)共5组(n=10),30 d处死,采用ELISA法测定血清Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)、透明质酸(HA)和层粘连蛋白(LN)浓度。结果:E组PCⅢ、Ⅳ-C、HA和LN测定结果依次为(57.96±11.22)、(30.69±9.86)、(170.96±49.21)和(75.58±13.47)ng.ml-1,与A组比较P<0.05,其他各组的测定结果无明显统计学差异。结论:小鼠肝脏植入125I粒子放射性活度≤37 MBq时,对肝纤维化指标无明显影响,125I粒子放射性活度达74 MBq时会引发各项肝纤维化指标的升高。

Effect of liver fibrosis on serum markers after 125I seed implanted into mouse liver

Objective:To observed the effect of liver fibrosis on serum markers after 125I seed implanted into mouse liver of different doses.Methods: 50 mice were randomly divided into control and 4 experimental groups of different doses of particles in(n=10).125I particle radioactivity order of 0,18.5,37.0 and 74.0 MBq,mice were sacrificed on day 30.Serum type Ⅲ procollagen(PC Ⅲ),Ⅳ collagen(Ⅳ-C),hyaluronic acid(HA) and laminin(LN) were detected by ELISA.Results: Serum type Ⅲ procollagen(PC Ⅲ),Ⅳ collagen(Ⅳ-C),hyaluronic acid(HA) and laminin(LN) in group E were respectively(57.96±11.22),(30.69±9.86),(170.96±49.21) and(75.58±13.47) ng·ml-1,significantly different from that in the control group P<0.05,but not from those in other experimental groups.Conclusions: A dose equal to or less than 37 MBq imposes no significant effect on liver fibrosis,however 74 MBq will increase the values of the indicators for fibrosis.