| 快速检测精子性染色体分析技术的建立 |
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| 引用本文： | 乐威,钱君海,赵华颖,袁涛,吴登龙,章劲夫.快速检测精子性染色体分析技术的建立[J].同济大学学报(医学版),2011,32(1):24-27. |
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| 作者姓名： | 乐威 钱君海 赵华颖 袁涛 吴登龙 章劲夫 |
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| 作者单位： | 同济大学附属同济医院泌尿外科,上海,200065 |
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| 基金项目： | 上海市科委基金资助项目(09411960100) |
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| 摘 要： | 目的建立一种应用双色荧光原位杂交技术快速分析正常男性精子XY染色体数目及异常率的方法。方法采用X、Y号染色体着丝粒探针对7例健康男性精子样本进行荧光原位杂交实验,检测精子XY染色体数目异常情况（非整倍体,双倍体）。结果计数10 078个精子,杂交率99.00%。其中X染色体杂交率为（49.789±1.346）%,Y染色体杂交率为（48.814±1.296）%,精子中双体类型为XX、XY、YY,其中XX杂交率为（0.110±0.053）%,XY杂交率为（0.222±0.077）%,YY杂交率为（0.094±0.038）%。结论采用荧光原位杂交技术建立了一种能够快速高效分析精子染色体数目异常的检测方法,可作为精子质量遗传学检测手段,应用于男性不育症的临床检测。
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| 关 键 词： | 双色荧光原位杂交 精子检测 非整倍体 双倍体 染色体数目畸变 |
Establishment of a rapid technical assessment of sex chromosome numerical abnormality in spermatozoa |
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| LE Wei,QIAN Jun-hai,ZHAO Hua-ying,YUAN Tao,WU Deng-long and ZHANG Jin-fu.Establishment of a rapid technical assessment of sex chromosome numerical abnormality in spermatozoa[J].Journal of Tongji University(Medical Science),2011,32(1):24-27. |
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| Authors: | LE Wei QIAN Jun-hai ZHAO Hua-ying YUAN Tao WU Deng-long and ZHANG Jin-fu |
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| Institution: | LE Wei,QIAN Jun-hai,ZHAO Hua-ying,YUAN Tao,WU Deng-long,ZHANG Jin-fu (Dept.of Urology Surgery,Tongji Hospital,Tongji University,Shanghai 200065,China) |
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| Abstract: | Objective To establish a rapid method to analyze the numerical abnormality of chromosome X and Y in spermatozoa from the healthy males by using two-color fluorescence in situ hybridization(FISH).Methods Fluorescence in situ hybridization was carried out by using centromeric probes targeting chromosomes,including X and Y upon the sperm samples harvested from 7 healthy men with normal semen quality.The frequency of numerical abnormality(aneuploidy,diploidy) was rated in each sample.Results A total of 10078 sp... |
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| Keywords: | two-color fluorescence in situ hybridization spermatozoa aneuploidy dipoidy chromosome numerical abnormality |
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