p53和p21WAF1蛋白在肝细胞肝癌中表达的意义

 【摘要】　目的　探讨p53和p21WAF1蛋白在肝细胞肝癌（HCC）中的表达及其意义。方法　应用免疫组织化学SP法检测41例HCC及相应30例癌旁肝组织中p53和p21WAF1蛋白的表达。结果　p53和p21WAF1蛋白在HCC中阳性表达率分别为43.9 ％和75.6 ％，明显高于癌旁组织，差异有统计学意义（P＜0.01）。HCC中p53蛋白表达与p21WAF1蛋白表达无明显相关性（P＞0.05）。p53蛋白表达在不同组织学分级、有无肝内或门静脉转移之间差异有统计学意义，而p21WAF1蛋白表达在不同临床病理特征间差异无统计学意义。结论　p53和p21WAF1蛋白高表达均在HCC发生、发展中起作用，但两者间并无相关性。p21WAF1的诱导存在着不依赖p53的途径。

Expression and significance of p53 and p21WAF1 proteins in hepatocellular carcinoma

【Abstract】　Objective　To explore the expression and significance of p53 and p21WAF1 proteins in hep－atocellular carcinoma(HCC). Methods　Immunohistochemical (S-P) method was used to detect the expression of p53 and p21WAF1 proteins in the 41 patients with HCC and 30 cases of paracancerous tissues. Results　The positive rates of p53 and p21WAF1 proteins were 43.9 % and 75.6 % respectively. The expression of the proteins was significantly higher in tumor than that in corresponding paracancerous tissue(P <0.01). No significant re－lationship was found between the expression of p53 protien and that of p21WAF1 in HCC(P ＞0.05). p53 ex－pression showed significant difference in different pathologic grades and cases with or without intrahepatic metastasis and thrombus in the portal veins(P <0.05), but p21WAF1 expression was not related to any clinico－pathological features. Conclusion　Both p53 and p21WAF1 overexpressions play a part in the pathogenesis of HCC, but there is no relationship between them. p21WAF1 expression in HCC might also be induced by p53-in－dependent pathway.