小柴胡汤对免疫抑制小鼠脾细胞凋亡的影响及其机制

Objective To explore the influence of Bupleuri Decoction on the apoptosis of splenic cells of immunosuppressed mice and its mechanism. Methods Normal mice and the immunosuppressed mice treated with cyclophosphamide were administrated Bupleuri Decoction for 10 days per day. Mean while normal mice and immunosuppressed mice were used as controls. At the 11th day, apoptosis of splenic-cells were detected with terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) assay. Expression of Bcl-2 and NF-κB of splenic cells were detected with immunocytochemistry. Results Compared with normal control, the splenic cell apoptosis increased (F =40. 65, P < 0. 01) and Bcl-2 as well as NF-κB expression decreased in the immunosuppressed mice (F = 37. 25, 52. 16, P < 0. 01). There was no obvious difference in the splenic cell apoptosis and expression of Bcl-2 and NF-kB between the normal control mice and the normal mice administered Bupleuri Decoction (F = 3. 76, 1. 69, 5. 48, P > 0. 05). Compared with immunosuppressed mice, the immunosuppressed mice administrated Bupleuri Decoction had lower splenic cell apoptosis and their expression of Bcl-2 as well as NF-κB increased obviously (F =36. 84, 29. 56, 37. 58, P <0. 01). Conclusion Bupleuri Decoction can modulating the immunological function of the immunosuppressed mice through modulating their splenic cell apoptosis.

Influence of Bupleuri Decoction on the apoptosis of splenic cells of immunosuppressed mice and its mechanism