Enzyme-linked immunosorbent assay for the detection of human rota virus in stools |
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| Authors: | Kathryn Bellamy P.S. Gardner M.H. Hambling S. Rice A.F. Bradburne |
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| Affiliation: | 1. DMRQC, Central Public Health Laboratory, Colindale, London, U.K.;2. Public Health Laboratory, LeedsU.K.;3. Queen Elizabeth Hospital for Children, Hackney, London, U.K.;4. Wellcome Research Laboratories, Beckenham, Kent, U.K. |
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| Abstract: | A simple method tor the detection of human rotavirus in stools is described, using a double antibody sandwich enzyme-linked immunosorbent assay. Polysterene microtitre plates were used as solid phase. Four capture antibodies were tried, bovine, egg-derived, guinea pig and monoclonal antibody to rotavirus. Both bovine and egg-derived antirotavirus labelled with horseradish peroxidase were used as the detecting antibodies. The results obtained were compared with a commercially available ELISA, Rotazyme (Abbott Laboratories), and also with the direct detection of rotavirus by electron microscopy. Bovine antibody was found to be an unsuitable capture antibody due to non-specific false positive reactions. |
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| Keywords: | ELISA rotavirus electron microscopy |
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