聚DL—天冬氨酸对庆大霉素耳毒性拮抗作用的实验研究

目的 研究聚ＤＬ－天冬氨酸（ＰＡＡ）对Ｆ－３４４大鼠庆大霉素（ＧＭ）耳毒性的拮抗作用。方法 选用健康Ｆ－３４４大鼠５０只，随机分４组：Ｉ为ＧＭ、Ⅱ为ＰＡＡ＋ＧＭ、Ⅲ为ＰＡＡ、Ⅳ为生理盐水对照组；通过观测４组大鼠不同时期、不同频率听性脑干反应（ＡＢＲ）阈值的改变；计数耳蜗毛细胞死亡率，以观察ＰＡＡ对Ｆ－３４４大鼠ＧＭ耳蜗毒性的拮抗作用；用双向扩散血清培养基检测法观察ＰＡＡ对ＧＭ抗菌活性的影响。结果

Protective effect of poly DL-aspartic acid on ototoxicity of gentamicin]

OBJECTIVE: To observe the protective effect of poly DL-aspartic acid (PAA) on ototoxicity of gentamicin (GM). METHODS: Fifty F-344 rats were divided into four groups, GM only PAA + GM, PAA only and saline control. ABR thresholds at different frequencies were measured at different times and hair cell losses were numerated. Two-dimensional diffusion assays in the culture medium were performed to evaluate the effect of PAA on the antimicrobial activity. RESULTS: Eighteen days after the treatment, ABR thresholds at 10 kHz and 8 kHz as well as hair cell losses in the GM-treated group showed significant differences as compared with other three groups (P < 0.01). CONCLUSION: PAA had the protective effect against the cochlear ototoxicity of GM without decreasing its antimicrobial activity.