| HIV检测基因芯片的研制和应用评价 |
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| 引用本文： | 朱京京,汪圣强,谢晓谦,钱盼盼,高杰,胡守旺. HIV检测基因芯片的研制和应用评价[J]. 现代医学仪器与应用, 2010, 0(1) |
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| 作者姓名： | 朱京京 汪圣强 谢晓谦 钱盼盼 高杰 胡守旺 |
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| 作者单位： | 无锡出入境检验检疫局;中山大学达安基因诊断中心; |
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| 基金项目： | 国家“十一五”科技重大专项(2008ZX10001-013); 国家质检总局科研项目(2008IK187) |
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| 摘 要： | 目的研制用于HIV检测的基因芯片,并评价其在临床诊断和出入境检疫中的应用价值。方法针对HIV-1gags基因相对保守区域,设计检测探针,并设计内标和对照探针,制备HIV检测微阵列基因芯片;设计引物,以1:20引物比例不对称扩增HIV基因靶标,Cy3荧光标记扩增产物,作为杂交模板。Cy3荧光标记PCR扩增产物与微阵列探针杂交反应,结果经荧光扫描,并用分型软件分析判断阳性探针和样本HIV阴阳性。采用信号放大、梯度临界值技术,增加了检测的灵敏度和特异性。采用70例临床样本实样检测与DNA测序作对照,评价试剂盒的性能和应用价值。结果70例样本,采用本试剂盒与DNA测序并行检测,两者符合率为98.57%。结论本HIV检测基因芯片试剂盒具有高通量、操作简便、低成本、准确度好等优点,应用研究表明,在临床HIV诊断检测和出入境检测方面具有很高的应用价值。
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| 关 键 词： | HIV 检测 基因芯片 DNA测序 应用评价 |
The preparation and application evaluation of DNA microarray genechip kit for HIV detection |
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| ZHU Jingjing,WANG Shengqiang,XIE Xiaoqian,QIAN Panpan,GAO Jie,HU Shouwang. The preparation and application evaluation of DNA microarray genechip kit for HIV detection[J]. , 2010, 0(1) |
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| Authors: | ZHU Jingjing WANG Shengqiang XIE Xiaoqian QIAN Panpan GAO Jie HU Shouwang |
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| Affiliation: | ZHU Jingjing1,WANG Shengqiang1,XIE Xiaoqian1,QIAN Panpan1,GAO Jie1,HU Shouwang2(1.Wuxi Entry-Exit Inspection , Quarantine Bureau,Jiangsu,Wuxi 214101,China,2.DaAn Gene Diagnostic Center of Sun Yat-sen University,Guangdong,Guangzhou 510665,China) |
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| Abstract: | Objective To develop a DNA microarray kit for HIV detection,and to evaluate the application value in clinical diagnosis and entry-exit inspection and quarantine.Methods The specific oligonucleotide probes for HIV-1 gags region and interior label or comparison probes were designed and spotted on glass slides using a microarray spotter.DNA sample were by amplified asymmetric PCR for HIV gags gene and the products of PCR were labeled with the fluorescent dye Cy3.The asymmetric PCR primer concentration ratio wa... |
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| Keywords: | HIV Detection DNAmicroarray DNASBT Application evaluation |
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