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| 3T3-L1前脂肪细胞分化过程中miRNAs表达谱的变化 | |
| 引用本文： | 凌宏艳,庹勤慧,胡弼,欧和生,刘刚,奉水东,朱炳阳,何剑琴,廖端芳.3T3-L1前脂肪细胞分化过程中miRNAs表达谱的变化[J].南华大学学报(医学版),2010,38(1):21-23,54. |
| 作者姓名： | 凌宏艳 庹勤慧 胡弼 欧和生 刘刚 奉水东 朱炳阳 何剑琴 廖端芳 |
| 作者单位： | 1. 南华大学,生理学教研室,湖南,衡阳,421001;南华大学,药理学教研室 2. 南华大学,药理学教研室 3. 南华大学,生理学教研室,湖南,衡阳,421001 4. 南华大学,流行病学教研室 |
| 基金项目： | 国家自然科学基金资助(30770868,30600249) |
| 摘 要： | 目的探讨3T3-L1前脂肪细胞分化过程中微小RNA(miRNAs)表达谱的变化。方法3T3-Ll前脂肪细胞培养和诱导分化,通过形态学观察和RT-PCR方法,判断3T3-Ll前脂肪细胞是否分化成脂肪细胞。运用miRNAs芯片技术检测3T3-Ll前脂肪细胞和脂肪细胞中差异表达miRNAs。结果3T3-L1前脂肪细胞呈现成纤维状,诱导分化的第9天,细胞变成球形,内含许多脂滴;过氧化物酶体增殖物激活受体r2(PPARr2)和脂肪性脂肪酸结合蛋白(aP2)的表达显著增加。3T3-L1前脂肪细胞分化成脂肪细胞上调的miRNAs有26个,下调的miRNAs有2个。结论3T3-L1前脂肪细胞分化过程中存在miRNAs表达谱的变化。 |
| 关 键 词： | miRNAs表达谱 3T3-L1前脂肪细胞 脂肪细胞分化 |
Differential Expression Profiles of miRNAs During 3T3-L1 Pre-adipocyte Differentiation |
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| LING Hong-yan,TUO Qin-hui,HU Bi,et al.Differential Expression Profiles of miRNAs During 3T3-L1 Pre-adipocyte Differentiation[J].Journal of Nanhua University(Medical Edition),2010,38(1):21-23,54. | |
| Authors: | LING Hong-yan TUO Qin-hui HU Bi |
| Institution: | Department of Physiology;University of South China;Hengyang;Hunan 421001;China |
| Abstract: | Objective To investigate the differentially expressed miRNAs in the procession of 3T3-L1 preadipocytes differentiation. Methods 3T3-L1 preadipocytes were cultured and induced to differentiate,the extent of adipocyte differentiation were assessed by microscopic observation and reverse trancription PCR(RT-PCR) methods.MicroRNA microarray was used to investigate the differentially expressed miRNAs in 3T3-L1 adipocytes and adipocytes. Results 3T3-L1 pre-adipocytes exhibied a fibroblast-like morphology which was... |
| Keywords: | miRNA expression profile 3T3-L1 preadipocytes adipocyte differentiation |
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