沙利度胺对人慢性粒细胞白血病细胞株K562细胞凋亡的影响研究

目的:研究沙利度胺对人慢性粒细胞白血病细胞株K562细胞凋亡的影响。方法:将K562细胞分为对照(未加药)组和沙利度胺低、中、高剂量(0·5、1·0、2·0mmol·L-1)组,加入相应药物分别作用24、48、72、96h后,MTT法检测各组细胞生长抑制率,Wright-Giemsa染色观察各组细胞形态变化,AnnexinⅤ-FITC/PI双染法流式细胞仪检测各组细胞凋亡率。结果:K562细胞的生长抑制率和凋亡率与沙利度胺浓度和作用时间均呈正相关;与对照组比较,沙利度胺3个剂量组作用72、96h后细胞的生长抑制率明显升高(P<0·05),4个时间点的细胞凋亡率均明显升高(P<0·01)。沙利度胺3个剂量组作用72h后,K562细胞出现细胞体积缩小等凋亡形态。结论:沙利度胺能够抑制K562细胞的增殖,呈一定的浓度和时间依赖性,并能够诱导K562细胞的凋亡。

Effects of Thalidomide on the Apoptosis of Human Chronic Myeloid Leukemia K562 Cell Line

OBJECTIVE:To study the effects of thalidomide on the apoptosis of human chronic myeloid leukemia K562 cell line.METHODS:K562 cells were divided into control(non-treated) group and thalidomide low-dose,medium-dose and high-dose groups(0.5,1.0,2.0 mmol·L-1).After 24 h,48 h,72 h and 96 h of treatment,MTT assay was used to determine the cell growth inhibition rate.Morphology of the K562 cells was observed by the Wright-Giemsa staining method.The rate of apoptosis was analyzed by flow cytometry(FCM) with AnnexinV-FITC/PI double-staining method.RESULTS:The growth inhibition rate and apoptosis rate of K562 cells were positively related with the concentration of thalidomide and treatment duration.Compared with control group,the growth inhibition rate of K562 cells in thalidomide groups increased significantly after 72 h and 96 h of treatment(P<0.05),and apoptosis rate of K562 cells increased significantly at 4 time points(P<0.01).After exposure to thalidomide for 72 h,K562 cells underwent typical morphological changes of apoptosis such as dwindling in size.CONCLUSIONS:Thalidomide inhibits the proliferation of K562 cells in a dose and time-dependent manner to some extent.Thalidomide obviously induces the apoptosis of the K562 cells.