Pyrazinamidase activity of Mycobacterium tuberculosis--a test of sensitivity to pyrazinamide |
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Authors: | S S Trivedi S G Desai |
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Institution: | Tuberculosis Research Centre, Gujarat, India. |
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Abstract: | Pyrazinamidase activity has been found to correlate with pyrazinamide sensitivity in strains of Mycobacterium tuberculosis. In vitro sensitivity to pyrazinamide in acidified L?wenstein-Jensen medium, and pyrazinamidase activity by the Wayne method, were determined in 378 clinical isolates of M. tuberculosis. A close correlation was observed between the results of both tests. This method of detecting pyrazinamidase activity was found to be a rapid, simple and reliable substitute for pyrazinamide sensitivity testing, and it overcomes the difficulty of growing M. tuberculosis at pH 5.5, as required in the standard method. |
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