| cAMP类似物对人恶性胶质瘤细胞BT_(325)增殖与分化的影响 |
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| 引用本文： | 陈奎生,尹先印,殷智榕. cAMP类似物对人恶性胶质瘤细胞BT_(325)增殖与分化的影响[J]. 中国实用神经疾病杂志, 2000, 0(1) |
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| 作者姓名： | 陈奎生 尹先印 殷智榕 |
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| 作者单位： | 河南医科大学病理教研室!郑州,450052,河南医科大学二附院神经外科!郑州,450003,河南医科大学病理教研室!郑州,450052 |
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| 摘 要： | 目的：观察cAMP类似物8－Br－cAMP对人恶性胶质细胞HT325增殖与分化的影响。方法：在人恶性胶质瘤细胞BT325中加终浓度为10－5mol／L的8－Br－cAMP体外培育24h后,应用酸解DNA及甲绿－派洛宁染色技术在同一标本上鉴别增殖型细胞和分化型细胞。结果：8－Br－cAMP处理组增殖型细胞占67％,分化型细胞占33％;而对照组增殖型细胞占85％,分化型细胞占15％。结论：8－Br－cAMP对BT325细胞有抑制增殖和促进分化效应。
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| 关 键 词： | 8－Br－CAMP 细胞增殖 细胞分化 BT_(325)细胞 |
Effects of cAMP analogues on proliferation and differentiation of human glioblastoma BT_(325) cells |
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| Chen Kuisheng, Yin Xianyin,Yin zhi rong. Effects of cAMP analogues on proliferation and differentiation of human glioblastoma BT_(325) cells[J]. Chinese Journal of Practical Neruous Diseases, 2000, 0(1) |
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| Authors: | Chen Kuisheng Yin Xianyin Yin zhi rong |
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| Abstract: | To observe the effects of cAMP analogs 8 - Br - cAMP on proliferation and differentiation of human glioblastoma BT325 cells. Methods: After the BT325 cells were cultured at final concentration of 10- 5 mol/L 8 - Br - cAMP for 24h, both proliferation and differentiation Pattern cells were identified on the same slide by using HCl hydrdysis of DNA and metlcyl green - pyronin stain technique . Results: The proliferation pattern cells decreased, while the differentiation pattern cells increased . Conclusion : The results suggest that 8 - Br - cAMP could facilitate BT325 cell differentiation and suppress BT325 cell proliferation. |
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| Keywords: | 8-Br -cAMP cell differentiation cell proliferation BT_(325)cells |
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