慢性肝炎,肝硬变和原发性肝癌内HBxAg的表达及意义

Expression and significance of HBxAg in human chronic hepatitis, cirrhosis and primary hepatic carcinoma

The specimens of this study consisted of 110 cases of chronic hepatitis, 108 cases of liver cirrhosis and 110 cases of primary hepatic carcinoma (PHC). Formalin-fixed and paraffin-embedded sections were stained by ABC method for HBxAg, and for HBsAg and HBcAg with PAP method. Of the 110 cases of chronic hepatitis, 72 (65.5%) showed HBxAg positivity in the liver cells, 66 (60.0%) were of HBsAg and 35 (31.8%) were of HBcAg. Among the 108 cases of liver cirrhosis, 84 (77.8%) revealed HBxAg positivity in liver cells, 73 (67.6%) were demonstrated to be of HBsAg-positive cases and 18 (16.7%) were shown to be of HBcAg-positive cases. Among the 110 cases of PHC, 64 (58.2%) showed the HBxAg-positive reaction in cancerous tissues, and 17 (15.5%) for HBsAg and 12 (10.9%) for HBcAg in hepatoma cells were confirmed; sixty-three (78.8%) of the 80 cases of the cancerous surrounding hepatic tissues displayed the HBxAg positivity, 47 (58.8%) for HBsAg and 21 (26.3%) for HBcAg were also positive. In this study, the detection rate of HBxAg in chronic hepatitis, liver cirrhosis and PHC was higher than that of HBsAg and HBcAg. This appearance was very obvious in hepatoma tissues. The results of this study suggested that a relationship between chronic hepatitis, liver cirrhosis, PHC and chronic persistent infection of HBV is very close.