| 巢—聚合酶链反应技术的改进 |
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| 引用本文： | 韩骅 清水章. 巢—聚合酶链反应技术的改进[J]. 医学争鸣, 1992, 13(5): 326-328 |
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| 作者姓名： | 韩骅 清水章 |
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| 作者单位： | 日本京都大学生物化学教研室(韩骅，王成济)，第四军医大学遗传子实验设施(清水章)，第四军医大学医化学部(本庶佑) |
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| 摘 要： | 作者改进了巢-聚合酶链反应技术.在第一轮聚合酶链反应(PCR)之后,进行低融点琼脂糖凝电泳.切下靶序列带或相应位置的凝胶,加热融化后稀释,再进行第二轮PCR.由于对第一轮反应的产物进行了电泳纯化,所以可进一步提高这种方法的特异性.用这种方法研究了转基因小鼠中抗体基因的表达.
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| 关 键 词： | 聚合酶链反应 基因 抗体 |
Improved nest-PCR method |
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| HAN Hua WANG Cheng-Ji Akira Shimizu and Tasuku Honjo. Improved nest-PCR method[J]. Negative, 1992, 13(5): 326-328 |
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| Authors: | HAN Hua WANG Cheng-Ji Akira Shimizu and Tasuku Honjo |
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| Affiliation: | HAN Hua WANG Cheng-Ji Akira Shimizu and Tasuku HonjoDepartment of Biochemistry Institute of Molecular Genetics and Department of MedicalChemistry,Kyoto University,Faculty of Medicine,Japan |
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| Abstract: | Nest-PCR was improved methodologically. The products of the first round of PCR were electrophoresed at low melting point agarose gel. The gel fragment containing the targeted band, either visible or invisible, was cut out from the gel, and then it was melted by heating for dilution. The second round of the reaction was carried out with this diluted gel solution as a template, and it should be more specific because the template had been purified by electrophoresis. The expression of the antibody gene in transgenic mice was investigated. |
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| Keywords: | polymerase chain reaction methods gene antibody |
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