Molecular characterization of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of bovine group B rotaviruses: identification of a novel VP4 genotype |
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| Authors: | Souvik Ghosh N Kobayashi S Nagashima M Chawla-Sarkar T Krishnan B Ganesh T N Naik |
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| Institution: | (1) Department of Hygiene, Sapporo Medical University School of Medicine, S 1, W 17, Chuo-Ku, Sapporo Hokkaido, 060-8556, Japan;(2) Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Tochigi, Japan;(3) Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India;(4) School of Biology, National Institute of Science Education and Research, Bhubaneswar, India |
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| Abstract: | Studies on bovine group B rotaviruses (GBRs) are limited. To date, only the VP6 gene of a single bovine GBR strain and the
VP7 and NSP5 genes of a few bovine GBR strains have been sequenced and analyzed. In the present study, using a single-primer
amplification method, we have determined the full-length nucleotide sequences of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes
of three bovine GBR strains from eastern India. In all six of these genes, the bovine GBR strains shared high genetic relatedness
among themselves but exhibited high genetic diversity with cognate genes of human, murine and ovine GBRs. Interestingly, as
with group A rotaviruses, the bovine GBR VP1, VP2, VP6 and NSP2 genes appeared to be more conserved than the VP4 and NSP1
genes among strains of different species. The present study provides important insights into the genetic makeup and diversity
of bovine GBRs, and also identifies a novel GBR VP4 genotype. |
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