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Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device |
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| Authors: | Bor-han Chueh Ying Zheng Yu-suke Torisawa Amy Y. Hsiao Chunxi Ge Susan Hsiong Nathaniel Huebsch Renny Franceschi David J. Mooney Shuichi Takayama |
| Affiliation: | (1) Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA;(2) Periodontics and Oral Medicine, University of Michigan, Ann Arbor, MI 48109, USA;(3) School of Engineering and Applied Science, Harvard University, Cambridge, MA 02115, USA;(4) Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA;(5) Macromolecular Science & Engineering program, University of Michigan, Ann Arbor, MI 48109, USA;(6) Present address: Department of Chemistry, Stanford University, Stanford, CA 94305, USA |
| Abstract: | This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel. |
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