急性钳夹型脊髓损伤模型大鼠造模方式的选择:一项网状Meta分析

目的:用动脉瘤夹创建急性钳夹型脊髓损伤模型是一种简单有效的造模方法.然而,为了建立这种急性钳夹型脊髓损伤动物模型,许多实验根据不同闭合力的动脉瘤夹和压迫时间采用了不同的造模方式,其中包括30 g压迫力的动脉瘤夹压迫脊髓60 s(30 g,60 s组);70 g压迫力的动脉瘤夹压迫脊髓30 s(70 g,30 s组);7...

Selection of modeling methods for acute compressive spinal cord injury:a network Meta-analysis

OBJECTIVE:Generating an acute compressive spinal cord injury model with an aneurysm clip is a simple and effective modeling method.However,to create the animal model of acute compressive spinal cord injury,many experimental studies have utilized diverse modeling methods according to different closure force aneurysm clips and duration,including aneurysm clipping with 30 g compression force to compress the spinal cord for 60 seconds(30 g,60 s);aneurysm clipping with 70 g compression force to compress the spinal cord for 30 seconds(70 g,30 s);aneurysm clipping with 70 g force to compress the spinal cord for 60 seconds(70 g,60 s);compressing the spinal cord to 1/2 of its diameter for 30 seconds by aneurysm clips(1/2,30 s).The present study aimed to assess whether there is a significant difference among these modeling methods,and to determine the method that is most suitable to establish the clamp-type spinal cord injury model.METHODS:A literature search of PubMed,Embase,CNKI,VIP,and WanFang databases was conducted for relevant studies from their inception dates to October 1,2019.The included literatures were initially screened according to the inclusion and exclusion criteria,and data extraction and quality evaluation were performed subsequently.The paired Meta-analysis,subgroup analysis,and network Meta-analysis were conducted to evaluate the effects of different modeling methods on the limb function of spinal cord injury rats.RESULTS:Fourteen articles with a total of 714 Sprague-Dawley rats were included in the study.All the included articles were scored 6-8 for quality evaluation.The Basso,Beattie and Bresnahan(BBB)scores of the four modeling methods in the 1st week after modeling ranked as follows:sham operation group>30 g,60 s group>1/2,30 s group>70 g,60 s group>70 g,30 s group.The BBB score in the 30 g,60 s group was significantly higher than that in the 1/2,30 s group,70 g,60 s group and 70 g,30 s group at 1 week postoperatively(P<0.05).Subgroup analyses suggested an association of locomotor recovery of spinal cord injury with the sex of rats.Differences in circadian activities of rats also affected the BBB scores.CONCLUSION:The four modeling methods described in the study can be used to create the animal model of acute compressive spinal cord injury.Comparison of the four modeling methods has revealed that the best way to create a clamp-type spinal cord injury model is to compress the spinal cord for 60-second aneurysm clipping with a closing force of 30 g.It is recommended to use female rats for modeling,but further research and verification are needed.