钛螺钉表面含硅二氧化钛纳米管层的体内成骨性能

背景:目前假体及骨植入材料多采用钛及其合金材料,该类材料具有优越的力学特性及优良的抗腐蚀性,但其生物活性不足,很难与骨组织之间形成良好的化学键合.目的:在纯钛螺钉表面制备含硅二氧化钛纳米管涂层,观察其体内促成骨性能.方法:利用阳极氧化法在纯钛螺钉表面制备二氧化钛纳米管层(阳性对照组),采用等离子体浸没离子植入和沉积法将...

In vivo osteogenic properties of silicon-incorporated titanium dioxide nanotubes on titanium screw surface

BACKGROUND:At present,titanium and its alloy materials are mostly used in prosthesis and bone implants.These materials have superior mechanical properties and excellent corrosion resistance,but their bioactivity is insufficient,so it is difficult to form a good chemical bond with bone tissue.OBJECTIVE:To prepare titanium dioxide nanotube coating containing silicon on the surface of pure titanium screw and observe its osteogenic properties in vivo.METHODS:Titanium dioxide nanotube layers were prepared on the surface of pure titanium screws by anodic oxidation method(positive control group).Silicon ions were deposited on the surface of titanium nanotube layers by plasma immersion ion implantation and deposition(experimental group).Pure titanium screws were used as negative control group.The morphology of each group was observed by scanning electron microscope.The three kinds of screws were implanted into the femur of SD rats.The femoral specimens were taken out at 2,4 and 6 weeks after implantation for biomechanical test,and immunofluorescence analysis was performed at 6 weeks after implantation.The animal experiment was approved by the Ethics Committee of Experimental Animal Management and Animal Welfare of Jiangnan University.RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that uniform double-layer nanotube arrays were observed in the experimental group and the positive control group,and there was no significant difference between the two groups.(2)At 2 and 4 weeks after screw implantation,the average peak pullout force of the experimental group was significantly higher than that of the positive control group and the negative control group(P<0.05),and the positive control group was significantly higher than the negative control group(P<0.05).At the sixth week,there was no significant difference in pullout force among the three groups(P>0.05).(3)Immunofluorescence showed that at 6 weeks after implantation,there was almost no new bone attachment around the screw in the negative control group,and extensive new bone attachment was observed around the screw thread in the experimental group and the positive control group,among which the new bone formation was the most in the experimental group.(4)The results showed that the silicon dioxide titanium nanotube layer could significantly promote the formation of new bone around the pure titanium screw and increase the pullout force of screws.