常氧与缺氧状态下脑啡肽和吗啡对兔肺动脉平滑肌增殖的调控作用

探讨常氧与缺氧状态下脑啡肽对兔肺动脉平滑肌增殖的调控作用及机制，为解决经皮腔内冠脉成形术支架后冠脉再狭窄问题提供新的理论依据。无菌取出新西兰白兔动脉段，在常氧和缺氧条件下胺照Ｒｏｓｓ贴片培养平滑肌，和相差显微镜，透射电镜和（－肌动蛋白体鉴定平滑肌细胞，四唑盐比色法及３－ＨｄＲ参人实验检测平滑肌的生长状况。

THE MODULATING EFFECT OF ENDOGENOUS OPIOID PEPTIDE AND MORPHINE ON PROLIFERATION OF PULMONARY ARTERIAL SMOOTH MUSCLE CELLS IN NORMAL NQGEN TENSION OR HYPOXIA CONDITION

To investigate the effect of endogenous opioid peptide and morphine on proliferation of pulmonary arterial smooth muscle cells under normoxic or hypoxic condition and analyze their mechanism, to provide the theoretical foundation for treating the restenosis of coronary artery after PTCA and stent. Rabbit pulmonary arterial smooth muscle cells are cultured in vitro, MTT method and 3H TdR incorporation were used. The proliferation of pulmonary arterial smooth muscle cells was markedly accelerated under hypoxia condition. L enkephaline significantly inhibited the proliferation of pulmonary arterial smooth muscle cells under normoxic or hypoxic condition, which could be reversed by naloxone. Morphine seemed to have no obvious effect on the proliferation and the DNA synthesis of pulmonary arterial smooth muscle cells. The restenosis of coronary artery after PTCA and stent was related to the depression of plasma L enkephaline level and the hypoxia of newly formed intima of the coronary artery.