连续核移植对异种山羊(Bore)克隆胚胎发育的影响

目的探讨连续核移植与异种山羊克隆胚胎发育之间的关系。方法分别以波尔(Bore)山羊耳部成纤维细胞、山羊-兔异种克隆桑椹胚卵裂球为核供体,以兔卵母细胞为受体,进行连续核移植。结果共构建145枚异种原代重构卵、73枚继Ⅰ代及20枚继II代重构卵,经电融合后,获得重构胚数分别为90、58和14枚,融合率分别为62.1%、79.5%和70%;162枚重构胚在同等条件下进行体外共培养,卵裂率分别为72.2%、75.9%和28.6%,囊胚率分别为10%、13.8%和0%;融合率方面,原代、继I及继II重构卵之间无显著差异(P>0.05);早期发育率无显著差异,但继Ⅰ代重构胚高于原代重构胚,相反,继II代远低于前两者,差异极显著(P<0.01)。结论波尔山羊体细胞核经第1次核移植过程中异种受体卵胞质作用后,比高度分化的体细胞更有利于重构胚的发育,但异种山羊克隆胚卵裂球反复多次暴露在异种卵胞质中,可能不利于细胞核的发育。

The effect of serial nuclear transfer on the development of goat (Bore) inter-species cloned embryo

Objective: To investigate the effect of serial nuclear transfer technology on the development of goat(inter)-species cloned embryo.Methods: Serial nuclear transfer was conducted with the goat(Bore) ear fibroblast cells(original group),the blastomere of goat-rabbit inter-species cloned morula(serial I and II group) as the donor cells and rabbit oocytes as the recipient cells.Results: The number of original,serial I and serial II reconstructed eggs was 145,73 and 20,respectively.By fusion and co-culture,90 original,58 serial I and 14 serial II reconstructed embryos were(obtained.) The cleavage rate reached 72.2%,75.9% and 28.6%,and the rate of development to blastocyst was 10%,13.8% and 0,respectively.There was no significant difference in the rate of fusion among(3 groups)(P>0.05).The early development rate of the serial I reconstructed embryos was slightly higher than that of original group.Eventually there were no serial II morula and blastocyst obtained and its early development rate was lowest in this study. Conclusions: The development potential of reconstructed embryo was improved with the somatic(nucleus) of goat exposed to the different species' cytoplasm as donor compared to high differential somatic cell.However,the effect could be possibly inverse by the blastomere of goat cloned embryo repeatedly treated in the different species' cytoplasm.