双抗体夹心ELISA检测循环抗原在血吸虫病控制流行地区应用的研究

对血吸虫病已控制流行地区1099名10岁以上的人群,应用抗血吸虫成虫抗原多克隆抗体(PcAb)为捕捉抗体,抗血吸虫虫卵抗原单克隆抗体(McAb)MG_2-HRP为覆盖抗体的双抗体夹心ELISA,检测血吸虫循环抗原(CAg),并以ELISA检测抗体(CAb)作平行对照.结果CAg和CAb的阳性检出率分别为4.55%和4.64%,两者的差异无显著意义(P>0.05).在50例CAg阳性者中,无血吸虫病治疗史者31例,而51例CAb阳性者中,无血吸虫病治疗史者仅6例.CAg和CAb联合检测能提高检出率.

APPLICATION ON THE DETECTION OF CIRCULATING ANTIGEN BY SANDWICH ELISA IN SCHISTOSOMIASIS CONTROLLED AREAS

A total of 1 099 sera specimens from people over 10 years of age in schistosomiasis controlled areas were detected. Circulating antigen was detected by sandwich ELISA using poly-clonal antibody and monoclonal antibody MGZ. At the same time,antibody in the same specimen was detected by ELISA. Results showed that the positive detecting rate of CAg and CAb were 4. 55% and 4. 64% , respectively. There was no significant difference between positive CAg and CAb (P>0. 05). 31 out of 50 patients were positive CAg but no history of treatment for schistosomiasis, while 6 out of 51 patients were positive CAb without history of treatment for schistosomiasis. The combined detection of CAg and CAb can raise the detection rate of schistosomiasis.