人C反应蛋白的发光免疫测定

本文叙述了人C反应蛋白(CRP)发光免疫的测定方法(LIA)。用6N-(4-氨基丁基)-N一乙基]-氨基-2,3-二氢吩噻嗪1,4-二酮(ABEI)标记羊抗兔IgG免疫球蛋白作为第二抗体,第一抗体是兔抗人CRP。固相载体以羊抗入CRP抗体包被。抗原抗体复合物的发光测定在LKB 1250型发光仪上进行。灵敏度为3.2ng/ml CRP,批间、批内变异系数分别为8.5％及4.6％。79名正常人血清CRP的平均值为1.82μg/ml。90％的正常值在2.6μg/ml以下。这些结果与放射免疫结果相似。26名患者血清同时进行LIA及火箭电泳测定,两法的结果相当符合,其相关系数(r)为0.94。

LUMINESCENCE IMMUNOASSAY OF HUMAN SERUM C REACTIVE PROTEIN

The method of luminescence immunoassay (LIA) of human serum C-reactive protein (CRP) is described in this paper. The immunoglobulin of sheep anti-rabbit IgG was labelled with 6 N-(4-aminobutyl)-N-ethyl]-amino-2, 3-dihydrophthalazine 1,4-dione (AB-EI) and taken as the second antibody. The first antibody used was rabbit antihuman CRP. The solid phase was coated with sheep anti-human CEP antibody. The luminescence of the antigen-antibody complex was measuered by LKB 1250 Luminometer, giving a sensitivity of 3.2ng/ml CRP with intra and inter assay coefficients of variation of 4.6% and 8.5% respectively. The mean value of the CRP of 79 normal human sera assayed by LIA was found to be 1.82μg/ml. Ninety percent of normal values is below 2.6μg/ml. These results were similar to that of the results assayed by RIA.The results from LIA of the sera from 26 patients were compared with that assayed by rocket immunoelectrophoreeie carried out simultaneously with the same samples. It was found that though all the data from LIA were lower than that assayed by the rocket im-munoelectrophoresis, yet the results obtained by these two methods correlated fairly well with a correlation coefficient (r) of 0.94.