| 中华仓鼠卵巢细胞在检测百日咳菌免疫原中的应用 |
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| 引用本文： | 王祖森,张蓉.中华仓鼠卵巢细胞在检测百日咳菌免疫原中的应用[J].现代免疫学,1992,12(4):204-207. |
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| 作者姓名： | 王祖森 张蓉 |
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| 作者单位： | 卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所，卫生部兰州生物制品研究所 兰州 730046，兰州 730046，兰州 730046，兰州 730046 |
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| 摘 要： | 采用中华仓鼠卵巢(CHO)细胞检测百日咳菌主要免疫原—百日咳毒素(LPF)和丝状血凝素(FHA),仅发现LPF使CHO细胞呈现明显的特异性簇聚反应,敏感性高,能检测出100pg/ml蛋白氮抗原量。与小鼠白细胞增多促进试验相比,CHO细胞法具有简便、经济、省时等优点,可作为一种检测百日咳毒素免疫原的实用辅助方法。
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| 关 键 词： | 卵巢细胞 百日咳菌 免疫原 仓鼠 |
THE APPLICATION OF CHINESE HAMSTER OVARY(CHO)CELL ON DETERMINATION OF BORDETELLA PERTUSSIS IMMUNOGEN |
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| Abstract: | Determining the action of the main immunogens, LPF and FHA, of Bordetella Pertussis on the CHO cells, we found a specific clustering response only by LPF. It was highly sensitive: concentration of the protein nitrogen (PN) of LPF antigen as low as 100 pg/ml could be detested. In comparison with the mice toxieity test in vivo,it was easy, cheap and time saving. It could be used as an adjuvant method for detecting the immunogen of pertussis toxin. |
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