双波长RP-HPLC同时测定黄连上清片中栀子苷和黄芩苷的含量

目的建立同时测定黄连上清片中栀子苷、黄芩苷含量的方法。方法采用双波长高效液相色谱法,色谱柱为Hibor C18柱（4.6 mm×150 mm,5μm）,流动相为乙腈-0.2%磷酸水溶液,梯度洗脱;栀子苷和黄芩苷检测波长分别为240 nm和278 nm;柱温为30℃。结果栀子苷和黄芩苷进样量分别在0.16～0.8μg（r=0.999 7）和0.24～1.2μg（r=0.999 8）内线性关系良好;平均加样回收率分别为95.7%和96.1%,RSD分别为2.63%和1.37%。结论方法简便、准确、重复性好,适用于黄连上清片中栀子苷和黄芩苷的含量测定。

Simultaneous Determination of Geniposide and Baicalin Content in Huanglianshangqing Tablets by Double Wavelength RP-HPLC

OBJECTIVE To establish an RP-HPLC method for simultaneous determination of geniposide and baicalin in Huanglianshangqing tablets.METHODS The analytical column was Hibor C18 column(4.6 mm×150 mm,5 μm).The mobile phase was composed of acetonitrile and 0.2% phosphoric acid with gradient elution.Geniposide and baicalin were detected at 240 nm and 278 nm,respectively.The column temperature was 30℃.RESULTS The calibration curves of geniposide and baicalin showed good linearity in the ranges of 0.16-0.8 μg(r=0.999 7) and 0.24-1.2 μg(r=0.999 8).The average recoveries were 95.7% and 96.1%.RSD were 2.63% and 1.37%.CONCLUSION The method is simple,accurate and reproducible for determining geniposide and baicalin in Huanglianshangqing tablets.