丙型肝炎患者肝特异性自身抗体的研究

目的:检测丙型肝炎患者血清肝特异性自身抗体并分析其临床意义.方法:以HEP-2细胞、猴肝、大鼠胃和大鼠肾组织为抗原,采用间接免疫荧光法对67例丙型肝炎患者血清、32例甲型肝炎、50例其它疾病和30例正常人血清作抗核抗体、抗平滑肌抗体、抗线粒体抗体和抗肝肾微粒体抗体检测.结果:丙型肝炎患者自身抗体总阳性率为25.4%,均高于其余3组对照组(P＜0.05).自身抗体以低滴度为主,主要为抗核抗体.自身抗体的阳性与患者的性别无关(P＞0.05),与年龄有关(P＜0.05).结论:HCV感染能诱导自身免疫反应使患者血清出现多种自身抗体,检测其自身抗体及其滴度对丙型肝炎患者的治疗有一定的参考价值.

Study on liver specific autoantibodies in patients with HCV

Objective:To study the clinical significance of determining serum liver specific autoantibodies in HCV patients.Methods:Hep-2 cells,rat liver tissue,rat kidney tissue and rat stomach tissue as substrates forANA,ASMA,AMA and ALKM were detected by indirect immunofluorescence assay in the serum of 67 HCV patients,32 HAV patients,50 patients with other diseases and 30 normal subjects.Results:The positive rate of autoantibodies in HCV patients were 25.4%,and there was significant difference compared with the other 3 groups( P <0.05).Low titer of autoantibodies was presented in HCV patients,and most of them were ANA.Autoantibodies were significantly associated with age( P <0.05),but not with gender( P >0.05).Conclusion:HCV infection can induce autoimmune reactions resulting in production of autoantibodies and its titer is helpful for treatment of HCV patients.