黄芪对糖尿病鼠肾脏氧化糖基化影响的实验研究

目的 探讨糖尿病肾病发病机理和探索黄芪治疗对糖尿病大鼠肾脏氧化糖基化的影响。方法 黄芪治疗四氧嘧啶糖尿病大鼠7周后，观察其对肾脏自由基代谢紊乱及非酶促糖基化的作用。结果 相关分析表明，血糖、果糖胺、肾组织脂质过氧化物丙二醛（MDA）与肾组织糖基化蛋白质呈显著正相关，超氧化物歧化酶（SOD）与肾糖化组织蛋白是明显负相关。黄芪治疗可明显提高糖尿病大鼠肾脏SOD活性，降低MDA及糖基化蛋白水平。结论 氧化糖基化是糖尿病肾病的重要发病基础，黄芪具有抑制氧化糖基化的作用。

The experimental study of the effect of Astragals Membrunceus Bge on nonenzymatic glycation and oxidation in kidneys of diabetic rats

Objective: To investigate the mechanism of diabetic chronic compli cations and the effect of Astragals Membrunceus Bge(AMB) on Alloaxan induced diabetic rats. Methods: Diabetic rats were treated with AMB for 7 weeks, and the effect of AMB on renal antioxidative enzyme activities and glycosylation was evaluated. Results: Correlative analyses showed that blood glucose, serum fructosamine, and maldondialdehyde were positively correlative to glycosylative protein of kidney (r=0 99, r=0 62, r=0 64, P<0 01), and negatively to superoxide dismutase (r=-0.42,P<0 05). AMB treatment enhanced the renal superoxide dismutase and reduced lipid peroxide levels and glycosylative protein in renal tissue. Conclusion: The experimental study suggests that the oxidative stress and nonenzymatic glycation of protein coexist in diabetes, and oxidative glycation forms the important basis of diabetic nephropathy. The therapeutic results indicate that AMB can inhibit the oxidative glycation of diabetic kidney, and the combination of AMB and insulin will act more effectively.