多参数流式细胞术在急性白血病免疫分型中的应用价值

目的探讨应用流式细胞术对急性白血病进行免疫表型的方法及临床意义。方法采用CD45/侧散射(SSC)双参数散点图设门方法进行三色流式细胞术细胞表面分化抗原的分析。结果79例白血病中,42例表达髓系抗原,以CD33表达最高,12例(28%)伴CD7表达,2例(5%)伴CD2表达,2例(5%)伴CD3表达,2例(2%)伴CD19表达;39例表达淋系抗原,以CD10表达最高,其中6例(6%)伴CD33表达,4例(10%)伴CD64表达;2例为双表达白血病。结论应用多参数流式细胞术能对急性白血病进行准确分型,这对白血病患者的治疗方案选择及预后判断等均有重要价值。

Application value of multi-parameter flow cytometry for immunophenotyping of acute leukemia

Objective It is to study the method and clinical significance of acute leukemia immunophenotyping by multi-parameter flow cytometry.Methods Immunophenotyping analysis was done by three color flow cytometry with the method of CD_(45)/SSC double parameter and scatter spot picture.Results In the 79 cases of leukemia,42 cases expressed medullary system antigen in which CD_(33) expression was the highest,12 cases(28%) with CD7 expression,2 cases(5%) with CD_2 expression,2 cases(5%) with CD_3 expression,2 cases(5%) with CD_(19) expression.39 cases expressed lymphocyte system antigen in which CD_(10) expression was the highest,6 cases(6%) with CD_(33) expression,4?cases(10%) with CD_(64) expression,2 cases was double expression leukemia.Conclusion Multi-parameter flow cytometry can accurately type acute leukemia,so it has an important value for the treatment choice and prognosis diagnosis of the patients with leukemia.