随机扩增多态DNA指纹技术的研究及在检测医院感染中的应用

目的 探讨医院感染常见病原菌的基因鉴定及分型方法，了解其分子流行病学情况。方法 优化随机扩增多态ＤＮＡ指纹技术（ＲＡＰＤ）的实验条件，利用ＲＡＰＤ技术成功地检测了儿科病房呼吸道合肥病毒（ＲＳＶ）、脑外科病房铜绿单胞菌（ＰＡ）及血液病房白色念珠菌（ＣＡ）的医院感染。同时进行了ＲＳＶ分离、ＰＡ菌和ＣＡ菌培养。结果 构建了金黄色葡萄球菌（ＳＡ）、志贺菌（ＳＢ）、大肠埃希菌（ＥＣ）、肠炎沙门菌（Ｓａｌｍ（

A randomized Study on amplified Polymorphic DNA Assay and its Application in Hospital Infection

OBJECTIVE To study hospital infection bacteria gene types and to know about their molecular epidemiology. METHODS Using RAPD technology to monitor Pseudomonas aeruginosa (PA) in neurosergical ward, Candidiasis albicans (CA) in cancer patients treated by chemotherapy and respiratory syncytial virus (RSV) in pediatric respiratory disease ward. RESULTS We found four PA strains, 21 CA straims; 49 RSV strains and there were four gene types, R 1 was the strain which caused hospital infection. RAPD compared isolating method gave the corcesponding rate 100% and the positive rate was higer than non isolating (P<0.01). CONCLUSION RAPD may be a good method to monitor nosocomial infection. It is specific and sensitive.