苯并芘氧化损伤鼠肺泡细胞及维生素E对其保护作用的机制研究

目的研究苯并芘（Benzo-a-pyrene,BaP）氧化损伤肺泡细胞的作用机制及探讨维生素E对其是否有保护作用。方法成熟雄性ICR小鼠100只随机分为A、B、C、D、E组,每组20只,A、B、C、D组每天BaP灌胃,B、C、D组同时用不同浓度维生素E灌胃,E组橄榄油灌胃,连续15 d后处死小鼠,取肺泡组织测氧化和抗氧化指标。结果 A、C、D组肺泡组织谷胱甘肽过氧化物酶（GSH-PX）、超氧化物歧化酶（SOD）活性明显高于A组（P〈0.05）,但低于E组（P〈0.05）;B、C、D组中肺泡组织活性氧（ROS）活力高于A、E组（P〈0.05）;B、C、D组中肺泡组织丙二醛（MDA）含量明显低于A组（P〈0.05）,B、C组高于E组（P〈0.05）。结论维生素E能抑制BaP所导致的肺泡组织GSH-PX、SOD活性降低与BaP所导致的肺泡组织MDA含量升高,维生素E能促进BaP所致的肺泡组织ROS活性升高。

Effort of Oxidative Damage on Alveolar Cells by Benzo-a-pyrene and the Machanism of Vitamin E Protection

Objective To study the mechanisms of action of oxidative damage on alveolar cells by benzo-a-pyrene（ BaP） and explore the protective effect of Vitamin E. Methods Viripotent male ICR 100 mice were randomly divided into group A,B, C,D and E,and every group included 20 mice. Mice in group A,B,C and D were lavaged with BaP,at the same time,and group B,C and D were lavaged with different concentrations of Vitamin E,while group E was lavaged with olive oil everyday. The mice were killed after continued lavage for 15 d,and then the oxydic and antioxidative indexes in alveolar tissue were detected. Results The activities of glutathion peroxidase（ GSH-PX） and superoxide dismutase（ SOD） in group B,C and D were significantly higher than those in group A（ P ＆lt;0. 05）,but lower than those in group E（ P ＆lt;0. 05）; The activities of reactive oxygen species（ ROS） in group B,C and D were significantly higher than that in group A and E（ P ＆lt;0. 05）; the malondialdehyde（ MDA） contents in group B,C and D were significantly lower than that in group A（ P ＆lt;0. 05）,and the contents in group B and C were higher than that in group E（ P ＆lt;0. 05）. Conclusion Vit E can inhibit the reduction of activities of GSH-PX and SOD and elevated MDA content induced by BaP,and it can also promote the elevated activity of ROS induced by BaP.