| 丹参药材脂溶性有效成分的快速指纹图谱研究 |
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| 引用本文： | 王敏,王逢春,郭琪. 丹参药材脂溶性有效成分的快速指纹图谱研究[J]. 华北国防医药, 2013, 0(11): 78-80 |
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| 作者姓名： | 王敏 王逢春 郭琪 |
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| 作者单位： | [1]北京军区总医院263临床部医务处,北京101149 [2]北京军区总医院医务处,北京100700 [3]北京军区联勤部药品仪器检验所,北京100071 |
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| 基金项目： | 军队中医药科研专项课题（10ZYZ204） |
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| 摘 要： | 目的建立丹参药材脂溶性有效成分的高效液相指纹图谱，为丹参药材质量控制提供可靠方法。方法选取10个产地的丹参生药材。色谱柱：AgilentSB—C18（2．1mm×50mln，1．8μm），流动相：甲醇-水（80：20），检测波长：254nm;进样量：20μl，流速：1．0ml／min。结果时间窗宽度为0．3min，共标定了11个共有色谱峰，各产地丹参药材的相似度一致性良好。结论本方法准确可靠，可为提高丹参药材质量控制标准提供参考。
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| 关 键 词： | 丹参 指纹图谱 色谱法 高压液相 |
A Study on the Quick Finger Printing of the Liposolubility Active Ingredient in Salvia Miltiorrhiza Bunge |
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| WANG Min,WANG Feng-chun,GUO Qi. A Study on the Quick Finger Printing of the Liposolubility Active Ingredient in Salvia Miltiorrhiza Bunge[J]. Medical Journal of Beijing Military Region, 2013, 0(11): 78-80 |
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| Authors: | WANG Min WANG Feng-chun GUO Qi |
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| Affiliation: | 1. Medical Department, 263 Clinical Department of General Hospital of Beijing Military Area Command, Beijing 101149, China; 2. Medical Department, General Hospital of Beijing Military Area Command, Beijing 100700, China; 3. Institute for Drug and Instrument Control, Combined Logistics Department of Beijing Military Area Command, Beijing 100071, China) |
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| Abstract: | Objective To establish an high-performance liquid chromatography （ HPLC ） finger printing of the liposolubility active ingredient in salvia miltiorrhiza bunge in order to provide a reliable method for the quality control of Salvia Miltiorrhiza Bunge. Methods Crude Salvia Miltiorrhiza Bunge from 10 different regions were collected. The chro- matographic column was Agilent SB-C18 （2. 1 mm × 50mm, 1.8 μm） , the mobile phase consisted of methanol-water （80 ： 20） , at 254 nm with 20 μl of injection volume for detection wavelength, and 1.0 ml/min of flow rate. Results The width of time window was set at 0.3 min, and 11 common chromatographic peaks were marked. Salvia Miltiorrhiza Bunge from different regions had good similarity and regularity. Conclusion The method is accurate and reliable, and can pro- vide a reference standard for the quality control of Salvia Miltiorrhiza Bunge. |
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| Keywords: | Salvia Miltiorrhiza Bunge Finger printing Chromatography, high pressure liquid |
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