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A novel method for diagnosis of smear-negative tuberculosis patients by combining a random unbiased Phi29 amplification with a specific real-time PCR
Affiliation:1. Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand;2. Office of Prevention Disease Control Region 10, Chiang Mai 50000, Thailand;3. Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand;4. Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand;5. School of Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand;1. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China;2. Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China;3. Division of Pulmonary Disease, Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu, China;1. Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia Romagna, National Reference Centre for Paratuberculosis, Strada Faggiola 1, loc. Gariga ‐ Podenzano (PC), 29027, Italy;2. Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia “Bruno Ubertini”, Food Control Department, Via Bianchi 9, 25124, Brescia, Italy;3. Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia “Bruno Ubertini”, National Reference Centre for Emerging Risks in Food Safety, Via Celoria 12, 20133, Milan, Italy
Abstract:
Keywords:Tuberculosis  Diagnostics  Smear negative  REPLI real-time PCR
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