Laparoscopic surgery in a small animal model |
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Authors: | B. A. Sandoval T. T. Sulaiman A. V. Robinson T. A. Stellato |
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Affiliation: | (1) Department of Surgery, University Hospitals of Cleveland, Case Western Reserve University, 11100 Euclid Avenue, 44106 Cleveland, OH, USA |
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Abstract: | Background: An adequate laparoscopic small-animal model would benefit surgical oncologic research. Immunobiologic data and reagents available for the rodent make them an ideal species. We developed a simple, inexpensive, reproducible technique for laparoscopic surgery in rodents.Methods: Carbon dioxide pneumoperitoneum is achieved in anesthetized animals. Through a 0.5-cm midline incision a 4.8-mm bronchofiberscope is inserted into the peritoneal cavity and secured with a purse-string suture (PSS). Three additional PSSs are made to introduce the dissectors. Under fiberscopic vision, a blunt dissection of the retroperitoneum exposes the inferior vena cava and aorta. Necropsy 24 h after verifies the adequacy of dissection.Results: Eighteen animals survived. The only death resulted from bleeding. Mortality was 5.26%. Surgical time was 24.72±8.93 min with all animals active 2 h postlaparoscopy.Conclusions: Laparoscopic surgery (LS) can be done inexpensively without sophisticated equipment. The rodent is ideal for examining the immunologic consequences of laparoscopic surgery and pneumoperitoneum. |
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Keywords: | Laparoscopic surgery Animal model Laparoscopic cholecystectomy Pneumoperitoneum |
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