替尼泊苷和洛莫司汀诱导脑胶质瘤细胞凋亡的机制

 目的 为探讨化疗药物替尼泊苷、洛莫司汀在脑胶质瘤化疗过程中诱导细胞凋亡的作用机制，以对其临床中的疗效进行有效监控。方法 取40例脑胶质瘤组织，分离培养胶质瘤细胞，加替尼泊苷和洛莫司汀，干预24、48、72 h时分别用流式细胞术（flow cytometre，FCM）测定脑胶质瘤细胞线粒体膜电位（mitochondrial membrane potential，MMP）、细胞周期和Bcl-2/Bax水平，并进行趋势性分析。结果 随着化疗药物干预时间的延长，MMP降低的比例迅速上升、细胞周期凋亡峰比例上升，Bcl-2表达下降，Bax表达大幅上升，Bcl-2/Bax比值下降。结论 替尼泊苷和洛莫司汀在脑胶质瘤化疗过程中诱导细胞凋亡的效果显著，VM-26对细胞周期的作用更强，在凋亡检测指标中MMP为最敏感、快捷的检测指标。

The role of cell apoptosis in glioma chemotherapy evaluation

Objective To study the role of cell apoptosis during the chemotherapy of human gliocytoma in order to get effective suvilliance on the effect of chemotherapy. Methods Gliocytoma cells were isolated and cultured from 40 human gliocytoma samples. Mitochondrial membrance potential (MMP), cell cycle, the level of Bcl-2 and Bax were detected by flow cytometry (FCM) at 24, 48, 72 hours respectively of incubation with Lomustine (CCNU) and Teniposide (VM-26), and the trends were also analysed. Results MMP decreased greatly, the apoptosis part in the cell cycle ananlysis increase, the expression level of Bcl-2 decreased, and that of Bax increase rapidly, while the Bcl-2/Bax ratio decreased. Conclusions CCNU and VM-26 have singnificant effect in gliocytoma chemotherapy on inducing gliocytoma cell apoptosis. VM-26 has more stronger effect on the cell cycle. MMP is the most sensitive and the fastest index in apoptosis detection.