烟酰胺在正常和气虚兔皮肤上促透作用的比较

目的:对比烟酰胺在正常和气虚家兔皮肤上的促透作用。方法:以烟酰胺为模型药在正常家兔与气虚家兔皮肤上进行透皮实验,测量不同时间接受液的药物浓度,计算其累积渗透量和渗透系数;制备病理切片,观察组织结构的变化。结果:与正常家兔离体皮肤的渗透系数比较,气虚家兔离体皮肤的渗透系数较低,二者比较有显著性差异(P<0.01)。病理组织切片显示,正常兔皮上皮细胞排列较为疏松,细胞间隙较大,上皮组织薄,脂肪等吸水性物质较多,脂肪层厚;气虚兔皮相对正常兔皮其上皮组织细胞排列紧密,脂肪较少。结论:正常家兔皮肤对烟酰胺的渗透性比气虚家兔皮肤更强。

Comparison of the Transdermal Absorption Effect of Nicotinamide between Normal Rabbit Skin and Skin of Rabbit Suffering from Qi-deficiency

OBJECTIVE： To compare the transdermal absorption of nicotinamide between normal rabbit skin vs. skin of rabbit suffering from qi-deficiency. METHODS： The transdermal absorption experimentation was carried out on the normal rabbits and rabbits suffering from qi-deficiency using nicotinamide as model drug. The drug concentrations of different times were measured, accumulative permeation amount and permeation coefficient were observed; Prepared pathological section was adopted to observe the change of skins structure. RESULTS： There was significant difference in permeation coefficient between normal rabbits skin and skin of rabbits suffering from qi-deficienc（P〈0.01）. Histopathological section showed epithelial cells of normal rabbit skin showed a more loose arrangement, big cell gap, thin epithelium, large amount of absorbent material such as fat, thick fat layer; compared with normal rabbit skin, epithelial cells of rabbit suffering qi-deficiency showed a close arrangement and thin fat layer. CONCLUSION： The transdermal absorption of nicotinamide in normal rabbit skin is superior to skin of skin of rabbits suffering from qi-deficiency.